Protective Effect of Schisandrin on Hydrogen Peroxide-Induced Damage in PC12 Cells

Abstract:

In this study, schisandrin was assessed for potential protective effects on pheochromocytoma cell line (PC12 cells). Using PC12 cells damage induced by H₂O₂(25μmol/L) as in vitro model. After pre-treatment with different concentration of schisandrin (0.3, 0.6, 1.2μM) for 24h, MTT assay was used to detect the cell viability, the supernatant of cells was collected to examine the levels of nitric oxide (NO) in each sample, and immunocytochemical staining was adopted to observe the expression levels of bcl-2. Results showed that schisandrin at different concentrations could increase the viability of PC12 cells and decrease the levels of NO in the culture medium. There were significant differences between schisandrin group and H₂O₂ group (P<0.05,P<0.01). Immunocytochemical staining result revealed that schisandrin could upregulate bcl-2 protein expression. In summary, schisandrin shown significant neuroprotective effects on H₂O₂-injured PC12 cells through antioxidant properties and upregulate bcl-2 protein expression, and could be a potential candidate for intervention in neurodegenerative diseases.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1545-1548
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1545
• Online since: August 2013
• Authors: Kuang Ren, Yan Chun Wang, Hong Yan Fan
• Keywords: Bcl-2, PC12, Schisandrin

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