Effects of Radix Platycodonis Polysaccharide on Expressions of IFN-γ, IL-4 and IL-5 in the Bronchoalveolar Lavage Fluid and Nf-κВ in the Lung Tissue of Asthmatic Rats

Abstract:

Objective: To investigate the effect of Radix playtycodoni polysaccharide (RPPS) on rats with ovalbumin-induced asthma and its mechanisms. Methods: 48 Wistar rats were randomly divided into blank control group, asthma model group, dexamethasone control group (1 mg / kg), and high-dose RPPS group (40 mg / kg), moderate-dose RPPS group (20 mg / kg) and low-dose RPPS group (10 mg / kg). The ovalbumin sensitization method was used to establish the asthma model in rats. The rats were given it in the atomization administration for two weeks and then were sacrificed. The BALF was collected for the count of eosinophils (EOS); enzyme-linked immunosorbent assay (ELISA) method was employed for the determination of IL-4, IL-5, and IFN-γ levels in BALF; the expression of NF-κВ in the lung tissues was measured by Western blot. Results: Compared with the model group, RPPS could reduce the number of EOS in blood and BALF, increase the content of IFN-γ in BALF, lower IL-4 and IL-5 contents, and down-regulate the expression of NF-κВ protein in rats with asthma. Conclusion: RPPS can inhibit the airway inflammation in asthma, down-regulate the expression level of IL-4 and IL-5, up-regulate the IFN-γ level to regulate the abnormal immune status induced by the imbalance of TH1/TH2 in asthma and inhibit the expression of NF-κВ, which may be one of the mechanisms of its anti-inflammatory activity.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1549-1554
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1549
• Online since: August 2013
• Authors: Xiao Dong Huang, Xing Yu Zhao, Yan Chun Wang*, Jian Ying
• Keywords: Asthma, Bronchoalveolar Lavage Fluid (BALF), IFN-γ, IL-4, IL-5, Nf-κВ, Radix Platycodonis Polysaccharides (RPPS)

References

[1] GelbAE, L icuanan J, Shinar CM, et al Unsuspected loss of lung elastic recoil in chronic persistent asthma [J]. Chest 2002, 121(3): 715-721.

DOI: 10.1378/chest.121.3.715

[2] Duncan CJ，Lawrie A，Blaylock MG，et al. Reduced eosinophil apoptosis in induced sputum correlates with asthma severity [J]. Eur Respir, 2003, 22(3): 484-490.

DOI: 10.1183/09031936.03.00109803a

[3] Gabriele G, Gerald J, Dale T, et al. Roles of interleuckin-13 and interferon-γ in lung inflammation [J]. Chest, 2002, 121(2): 88-95.

[4] Taniguchi M, Takenchi T, Nakatsuka R，et al. Molecular process in acute liver injury and regeneration induced by carbon tetrachloride [J]. Life Sci. 2004; 75(13): 1539-1549.

DOI: 10.1016/j.lfs.2004.02.030

[5] Wang Yanni, ChangXiaohong. Th1/Th2 imbalance and bronchial asthma [J]. Medical Information, 2011, 24(6): 390.

[6] Wang Xiaoyan, Chi Baorong, Chen Lu. Effects of Chuanxikang decoction on peripheral plasma levels of IFN-γ, IL-5, Eos and Eotaxin in bronchial asthma rat [J]. J Fourth Mil Med Univ, 2008, 29(8): 682-684.

[7] Du Qiang, Hang Mao. Effects of tripterygium polyglucoside on the expression of ICAM-1 and NF-κB in the bronchioles of asthmatic rats. Acta Universitatis Medicinalis Nanjing (Natural Science), 2005, 25(4): 252-254.