A New Host-Guest Biomaterial Apply for DNA Sequence Electrochemical Detection

Abstract:

In this paper, a novel dually-labeled DNA probe (DLP) in a stemloop structure was designed to be labeled at one end with dabcyl, working as a guest molecule, and at its another end with CdS nanoparticle, working as an electrochemical tag to indicate the electrochemical signal of hybridization occurrence. One a-cyclodextrin/multi-walled carbon nanotube modified glassy carbon electrode (a-CD/MCNTs/GCE) was fabricated for the hybridizer capture. The DLP could recognize target DNA through hybridization event between complementary bases in the loop of probe DNA and target DNA. Without binding with target DNA, the DLP kept its stemloop structure which held dabcyl molecule shielded by the loop of probe DNA and CdS nanoparticle. This structure blocked dabcyl from entering the cavity of a-CD on the electrode. Once hybridization happened, the stem-loop structure of hairpin DNA opened and then dabcyl molecule could be easily captured by a-CD on the electrode. Thus, the electrochemical response of the CdS nanoparticle label at the other end of the hybridizer was proportional with the concentration of target DNA.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1495-1498
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1495
• Online since: August 2013
• Authors: Hao Fan*, Cui Han Feng, Yan Lin
• Keywords: Cyclodextrins, DNA, Double-Labeled Probe, Electrochemical, Homogeneous

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