Selective Breeding of Oxygen-Tolerant and Oxalate-Degrading Lactic Acid Bacteria by Protoplast Fusion

Abstract:

Bifidobacterium lactis with oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and it can be used to prevent and treat kidney stone diseases. While Bifidobacterium lactis is poorly oxygen-tolerant, which hinders it from being as microbial ecological agents. To obtain oxygen-tolerant and oxalate-degrading lactic acid bacteria, protoplast fusion technology was used between B. lactis and L. acidophilus. Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, the fusion time 7 min, the fusion temperature 30°C, the concentration of CaCl₂ 0. 02mol/ L and the concentration of MgCl₂ 0.5mol/ L, the fusion rate reached 7.6%, and three oxygen-tolerant fusant showing that the level of oxalate degradation were similar with B. lactis was obtained. The fusants of SZY1-7 and SZY2-1 could tolerance to pH 2.5 and 0.5% (w/v) bile salt.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1489-1494
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1489
• Online since: August 2013
• Authors: Sheng Chen*, Yu Li, Wen Bin Jin, Yan Chen, Xiao Guang Liu, Fu Ping Lu
• Keywords: Bifidobacterium lactis, Lactobacillus acidophilus, Oxalate-Degrading, Protoplast Fusion

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