Effect of Camptothecin on the Embryonic Development and Angiogenesis of Zebrafish Embryos

Abstract:

Camptothecin (camptothecin, CPT) is a kind of anti-cancer drugs targeted at DNA topoisomerase with strong toxicity and side effects. The zebrafish is a model organism to study the anti-tumor drugs. The CPT was added into culture system in early development period of zebrafish embryos, and staining techniques were used to study embryonic development and angiogenesis in zebrafish. Results showed that low concentration of CPT can affect embryonic development seriously, and trigger the developmental delay which might led to teratism or death and accompanied with apoptosis phenomenon at the same time. Besides, CPT can also inhibit the development of intestinal vein in zebrafish embryos, which provided the experimental basis for the research of the pharmacological analysis, screening and evaluation model of topoisomerase I inhibitor class drugs.

• Periodical: Advanced Materials Research (Volumes 750-752)
• Pages: 1472-1475
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.750-752.1472
• Online since: August 2013
• Authors: Chun Xia Song, Shu Liang Song, Hao Liang, Xu Liu
• Keywords: Angiogenesis, Camptothecin, Embryonic Development, Zebrafish

References

[1] C.J. Li, Y. Gu, Y.X. Han, et al. Chin J Med Chem, Vol. 13 (2003), p.306.

[2] H.M. Stern, L. Zon. Nat Rev Cancer, Vol. 3 (2003), p.533.

[3] J.F. Amatruda. Cancer Cell, Vol. 1 (2002), p.229.

[4] S.L. Yang, L.H. Zheng, X.K. Lin, et al. Chinese High Technology letters, Vol. 17 (2007), p.23.

[5] L.H. Zheng, Ling P, Wang Z, et al. Cancer Biol Ther, Vol. 6 (2007), p.775.

[6] Wang Z, R.L. Niu, X.K. Lin, et al. Biochem Biophys Res Commu, Vol. 357 (2007), p.26.

[7] U Langheinrich, E Hennen, G Stott, et al. Curr Biol, Vol. 12 (2002), p.2023.

[8] X Zhao, N Wu, L Ding, et al. FEBS Lett, Vol. 586 (2012), p.1220.