Optimizing the Conditions for Polysaccharides Ultrasonic-Assisted Extraction in Mycelium of Paecilomyces Tenuipes Pt196 Using Statistical Approach

Abstract:

In order to enhance the extraction rate of polysaccharides, a series of statistical approach was used to optimize the ultrasonic-assisted extraction conditions from the mycelium of Paecilomyces tenuipes Pt196. [Metho The optimization of conditions was carried out in two stages. Firstly, the effects of various experimental parameters considered for the investigation (ultrasonic power, ultrasonic time and liquid-solid ratio) were studied using the method of single factor test design experiments. Secondly, a 15-run Box-Behnken design was performed to optimize the extraction conditions of polysaccharides. The experimental results of Box-Behnken design were analyzed by response surface methodology and artificial neural network together with genetic algorithm. [Result The optimum conditions for polysaccharides extraction obtained by the application of artificial neural network-genetic algorithm were ultrasonic time 345s, ultrasonic power 320 W and liquid-solid ratio 95 mL·g-1. [Conclusion Artificial neural network-genetic algorithm can effectively select the best extraction conditions of polysaccharides in this study.

• Periodical: Advanced Materials Research (Volumes 756-759)
• Pages: 180-185
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.756-759.180
• Online since: September 2013
• Authors: Lin Na Du, Wen Ting Bu, Wen Lin Pan, Chen Shao, Jia Hui Lu, Qing Fan Meng, Li Rong Teng
• Keywords: Artificial Neural Network (ANN), Genetic Algorithm (GA), Paecilomyces Tenuipes, Polysaccharide, Response Surface Methodology (RSM)

References

[1] C.P. Xu, S.W. Kim, H.J. Hwang, and J.W. Yun, Production of exopolysaccharides by submerged culture of an enthomopathogenic fungus, Paecilomyces tenuipes C240 in stirred-tank and airlift reactors, Journal of Bioresource Technol., vol. 97, pp.770-777, (2006).

DOI: 10.1016/j.biortech.2005.01.042

[2] H.C. Kim, B.S. Choi, K. Sapkota, S. Kim, H.J. Lee, J.C. Yoo, and S.J. Kim, Purification and characterization of a novel, highly potent fibrinolytic enzyme from Paecilomyces tenuipes, Process Biochem., vol. 46, pp.1545-1553, (2011).

DOI: 10.1016/j.procbio.2011.04.005

[3] T. Takata, T. Tanaka, N. Yahagi, R. Yahagi, H. Tsuchida, Y. Ishigaki, N. Tomosugi, S. Fushiya, F. Takano, and T. Ohta, The liquid culture filtrates of entomogenous fungus Paecilomyces tenuipes and its glycoprotein constituent protects against Anemia in Mice Treated with 5-fluorouracil, Biol. Pharm. Bull, vol. 31, pp.1565-1573, (2008).

DOI: 10.1248/bpb.31.1565

[4] C.P. Xu and J.W. Yun, Influence of aeration on the production and the quality of the exopolysaccharides from Paecilomyces tenuipes C240 in a stirred-tank fermenter, Enzyme Microb. Tech., vol. 35, pp.33-39, (2004).

DOI: 10.1016/j.enzmictec.2004.03.012