Numerical Simulation and Experimental Study on Light Scattering by Biological Cells with Discrete Dipole Approximation

Abstract:

An experimental apparatus for the analysis of biological cells light scattering in liquid suspensions has been presented. Characterization is based on the scattering of a monochromatic laser beam by particles [which can be inorganic, organic, or biological (such as animal cells and bacteria)] and on the strong relation between the light-scattering pattern and the morphology and refractive index of the particles. In order to study light scattering in biological cells close to the actual situation, we focus on non-spherical particles in the cell-culture medium. Finally, we demonstrate the light scattering results of bovine kidney cells suspended in the cell-culture medium, and compares then with the simulated results.

• Periodical: Advanced Materials Research (Volumes 760-762)
• Pages: 105-109
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.760-762.105
• Online since: September 2013
• Authors: Jian Bin Liu, Hai Li, Ying Xin Zeng, Jia Wen Weng, Chu Ping Yang
• Keywords: Biological Cells, Discrete Dipole Approximation (DDA), Light Scattering, Non-Spherical Particles

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