Study on Enzymatic Synthesis of γ-Cyclodextrin with β-Cyclodextrin

Xin Zhi Cao; Jian Ming You; Kai Bin Lv

doi:10.4028/www.scientific.net/AMR.774-776.901

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 774-776Study on Enzymatic Synthesis of γ-Cyclodextrin...

Study on Enzymatic Synthesis of γ-Cyclodextrin with β-Cyclodextrin

Abstract:

Gamma cyclodextrin was produced synthesized from beta cyclodextrin in the presence of glycyrrhizin, using Cyclodextrin glucanotransferase (EC 2.4.1.19) from Alkalophilic Bacillus. The results showed that more than 32.7% of beta cyclodextrin substrate was converted to the corresponding gamma cyclodextrin under the following conditions, viz. 200 units enzyme/g of β-CD, 10mmol/L of β-CD concentration, 3% of glycyrrhizin concentration, 27 h of reaction time, 65°C of temperature and a reaction pH of 7.0.