Fermentation Condition of Penicillium purpurogenum HBZ003 from Mangrove for 1,4-β-D-cellobiohydrolase Activity

Abstract:

The cellulolytic enzymes from Penicillium genus showed well-balanced amounts of cellobiohydrolase, endo-cellulase and β-glycosidase. The Fermentation condition of Penicillium purpurogenum HBZ003 from Mangrove for 1,4-β-D-cellobiohydrolase activity was investigated. The optimum fermentation condition of Penicillium purpurogenum HBZ003 was as follows. The medium was composed of 1.6% bran, 0.4% CMC, 0.5% (NH₄)₂SO₄, 0.4% KNO₃, 0.6%NaCl, 0.03% CaCl₂ and 0.05% Tween 60, and adjusted to initial pH 4.0, and inoculated with 10% seed and cultivated at 160 r/min and 30 °C for 5d. The 1,4-β-D-cellobiohydrolase activity reached 5.54 U with the ratio of 1,4-β-D-cellobiohydrolase, endo-cellulase and β-glycosidase activity being 1:3.26:5.09.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1284-1288
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1284
• Online since: September 2013
• Authors: Yi Duo Wang, Cong Fa Li, Si Xin Liu
• Keywords: 1,4-β-D-Cellobiohydrolase, Fermentation Condition, Mangrove, Penicillium purpurogenum

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