Establishment of Micellar Electro Kinetic Chromatography Detection Method for Puerarin in Pueraria Lobata(Willd.)Ohwi

Abstract:

Relative to the pharmacopoeia of high performance liquid chromatography (HPLC), establishing detection of puerarin in radix puerariae micellar electro kinetic chromatography (MEKC). Studying on buffer pH, borax concentration, separation voltage, SDS concentration of chromatographic conditions on the puerarin MEKC separation effect of influence, determined the best capillary electrophoresis conditions: test 250 nm wavelength, buffer of pH 9.0 0.1M KH₂PO₄ solution-20 mM borax solution, separation voltage 18 kV, 40 mmol/L SDS concentration, 4.5 min retention time., grained puerarin MEKC standard equation: Y=36.522X-471.9, R²=0.9994(N=5), the puerarin concentration in 550 μg/mL to 750 μg/mL range comes out a good relationship. Get the lowest detection limit with 13.74 μg/mL. Puerarin content of the total isoflavone detected by MEKC was 45.35% that similar to 42.25% detected by HPLC. The method was stable, accurate, sensitive, possessed organic solvent consumption. The research results have applications in puerarin and its product quality evaluation system and promote isoflavonoids detection.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1289-1293
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1289
• Online since: September 2013
• Authors: Xing Rong Liu, Dao Chen Zhu, Yuan Mei Chen
• Keywords: Detection, High Performance Liquid Chromatography (HPLC), MEKC, Pueraria lobata (Willd)Ohwi, Puerarin

References

[1] S.H. Kim, et al. Phytother. Res., Vol. 23(2009), p.701.

[2] E. Meezan, et al. J. Agric. Food Chem., Vol. 53(2005) No. 22, p.8760.

[3] Y. Yang, et al. Journal of Clinical Rehabilitative Tissue Engineering Research, Vol. 32(2009) No. 13, p.6388. (In Chinese).

[4] X.L. Shen, et al. Journal of Hainan Medical College, Vol. 10(2004) No. 3, p.136. (In Chinese).

[5] R. Adam, et al. J. Agric. Food Chem., Vol. 56(2008) No. 17, p.7860.

[6] J. Vacek, et al. J. Sep. Sci., Vol. 31(2008) No. 11, p. (2054).

[7] X.R. Liu, et al. Food Research and Developent, Vol. 27(2006) No. 10, p.126. (In Chinese).

[8] X.R. Liu, et al. Jiangsu Agric. Sci. Vol. 41(2013) No. 2, p.273. (In Chinese).

[9] D. Constantine and Stalikas. J. Sep. Sci., Vol. 30(2007) No. 18, p.3268.