The Study of the Kinetic Model of Halomonas Salina Fermenting Ectoine

Shuang Gao; Ling Hua Zhang; Qing Chen; Lin Bai; Ya Jun Lang

doi:10.4028/www.scientific.net/AMR.781-784.647

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The Study of the Kinetic Model of Halomonas Salina Fermenting Ectoine
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784The Study of the Kinetic Model of Halomonas Salina...

The Study of the Kinetic Model of Halomonas Salina Fermenting Ectoine

Abstract:

Ectoine had important physiological functions and superior potential applications, so the study of ectoine was extensively attented. This article was related to kinetic models of cell growth, product formation and substrate consumption, which was not only established according to the characteristics of ectoine batch fermentation by Halomonas salina DSM 5928 but also obtained the kinetic parameters by the nonlinear fitting method in the Microcal Origin software. Logistic, Luedeking-Piret and Luedeking-Piret-like equations was applied to analyze the cell growth, the ectoine formation and the substrate consumption by the kinetic model，respectively. The results between calculated values and experimental data were coincident. By fitting, correlation coefficients R2 were ≥ 0.989. The fermentation conditions of ectoine were analyzed according to the model. The results showed that ectoine productivity (0.28 g/L/h) was the highest when initial monosodium glutamate concentration (S0) was 60 g/L. However, when S0 was 80 g/L, the ectoine concentration was maximal, i.e., 7.59 g/L. The research suggested that ectoine formation belonged to the mixed kinetic mechanism of cell growth and biomass concentration, while the ectoine production mainly depended on instantaneous biomass concentration. The fermentation method for improving ectoine concentration was further proved. The established kinetic model will be of significant value to provide the optimal conditions of present process.