Ultrasound-Microwave Assisted Extraction and Separation of Ricinus communis Allergen from Castor Bean Meal

Ai Lin Zhang; Chang Lu Wang; Yu Feng Hu; Zhi Jiang Zhou

doi:10.4028/www.scientific.net/AMR.781-784.721

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Ultrasound-Microwave Assisted Extraction and...

Ultrasound-Microwave Assisted Extraction and Separation of Ricinus communis Allergen from Castor Bean Meal

Abstract:

The influence of ultrasound-microwave during extraction of Ricinus communis(L）allergen from castor bean meal (CBM) was investigated. The method was successfully applied for the ultrasound- microwave assisted extraction (UME) of allergen from CBM. The corresponding extraction parameters including the extraction temperature, ultrasonic-microwave power and extraction time were investigated. The CBM was crushed, sifted, ground and suspended for 4 h in 25% ethanol water (1:4) at room temperature followed by ultrasound-microwave. The yield, polysaccharose and protein contents were determined after each processing step. The purity of extracted allergen was tested through HPLC and the Sephadex G-100 and Tricine-SDS-PAGE detection with unique electrophoresis strip documented that allergen was purifed.. The paper describes the conditions of the temperature,time,and microwave-power for the method reliability with UME and suitablilty for the effective extraction of allergen from CBM.

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Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

721-725

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.721

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Online since:

September 2013

Authors:

Ai Lin Zhang*, Chang Lu Wang, Yu Feng Hu, Zhi Jiang Zhou

Keywords:

Allergen, Castor Bean Meal, Ricinus Communis L, Ultrasound-Microwave Assisted Extraction & Separation

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