A Bio-Material of Polymer Beads Based on Antibody Immobilization via Oxidized Glycochains Linkage for Flow Cytometric Suspension Array

Sha Li; Lai Yang Li; Jie Li; Lei Ji Zhou

doi:10.4028/www.scientific.net/AMR.781-784.694

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784A Bio-Material of Polymer Beads Based on Antibody...

A Bio-Material of Polymer Beads Based on Antibody Immobilization via Oxidized Glycochains Linkage for Flow Cytometric Suspension Array

Abstract:

A preparation of a bio-material of polymer beads used for immuno-assay is presented, in which the probe antibody is site-specific immobilized on the polystyrene microbead by the surface hydrazide activation and the oxidized glycochains linkage of antibody. Using adipic dihydrazide, free hydrazide-bearing matrix was acquired on carboxyl polystyrene microbead. An iron (III)-phenanthroline spectrophotometric method was developed to evaluate the activation efficiency. The dependence of probe antibody oxidization on the reaction time and the oxidant concentration was investigated. Also, an alternative strategy of antibody immobilization via traditional COOH/-NH₂ linkage was illustrated for a comparison with this site-specific immobilization in the aspects of the total antibody binding amount and the site-specific binding amount. The results showed that the site-specific efficacy using glycochains linkage-based binding was three times better than that using traditional amino-based binding, which indicated a higher sensitivity for application in immuno-assay.

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Periodical:

Advanced Materials Research (Volumes 781-784)

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694-698

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.694

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Online since:

September 2013

Authors:

Sha Li, Lai Yang Li, Jie Li, Lei Ji Zhou*

Keywords:

Antibody Immobilization, Oxidized Glycochains Linkage, Suspension Array

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