Research on Antibacterial Activity of Crude Extract from Mangifera Indica Leaves

Abstract:

The 95% ethanol extracts of mangifera indica leaf was sequentially extracted with petroleum ether, ethyl acetate, n-butyl alcohol and water. Antibacterial activity and minimal inhibitory concentration (MIC) of the four different extraction against Staphylococlus aureus, Escherichia coli, Bacillus subitlis and Saccaromyces cerevisiae were stuied with filter paper method in this paper. Each of the four extracts showed different antibacterial activity: The water extract is the strongest one; l-butanol extract takes second place; Ethyl acetate and petroleum ether extract is the third. MIC of aqueous extract with the strongest inhibitory effect on Staphylococlus aureus, Escherichia coli and Saccaromyces cerevisiae is 0.0125 g/mL, 0.0125 g/mL, and 0.025 g/mL. Moreover, the aqueous extract showed powerful antibacterial activity against all of four bacteria.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 760-763
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.760
• Online since: September 2013
• Authors: Shao Juan Jiang, Ying Zhang
• Keywords: Antibacterial Activity, Filter Paper Method, Mangifera Indica Leaf, Minimal Inhibitory Concentration

References

[1] Liu Xueping, Jiang Weizhe, Huang Xingzhen, et al. Study on Antibacterial Action of Extracts of Leaves of Mangifera indica in Vitro[J]. China Pharmaceuticals, 2007, 16(9): 12-13.

[2] XIE Sandu, ZHENG Shengxuan, LUO Xiaoqing, Extraction of Flavonoids from Mango Leaves and Their Antibacterial Effect[J]. Chinese Journal of Tropical Crops, 2012, 33(12): 2257-2261.

[3] Deng Jiagang, FENG Xu, WANG Qin, et al. Study on the content of Mangiferin in Mangifera indica L. from different areas of Guangxi[J]. Chinese Traditional Patent Medicine, 2006, 28: 12: 1755-1756.

[4] Wei Naiqiu, Deng Jiagang, Xian Hanmei, et al. Comparison Tests of the Efficacy of Mango Leave Decoction, Demangiferin Mango Leave Decoction and Mangiferin Anti-tussive and Expectorant Drugs[J]. Henan Traditional Chinese Medicine, 2009, 29(1): 42-44.

[5] WEI Ying-fang, LIAO Lan-yan, LIN Jie, et al. The extraction of mangiferin from mango leaves and its analgesic function[J]. Journal of Youjiang Medical College for Nationalities, 2008, 30(1): 15-16.

[6] Hu Yanjun, Liu Shen, Wang Dingyong. Chemical Constituents of the Leaf of Mangifera indica[J]. Asia-Pacific Traditional Medicine, 2010, (2): 18-19.

[7] QIU He-yuan, LIN Mu-xi, WANG Yan-chun. Study on ultrasound-assisted extraction technology of flavonoids from Mangifera indica L. [J]. Guangdong Agricultural Sciences, 2011, (3): 80-82.

[8] Ping Shen, Guanwu Li. Microbiology experiment[M], Beijing: High Education Press, 2004: 50-54.

[9] XIE Sandu, ZHENG Shengxuan, LUO Xiaoqing, Extraction of Flavonoids from Mango Leaves and Their Antibacterial Effect[J]. Chinese Journal of Tropical Crops, 2012, 33(12): 2257-2261.