Effect of High Pressure Microfluidization on Secondary Structure of Wheat Gluten in Different Solvents

Abstract:

Far-UV circular dichroism (CD) spectroscopy was used to study the conformation of wheat gluten protein treatmented by dynamic high pressure microfluidization (DHPM), acid treatment and its comprehensive treatment in two solvents. The results showed, the secondary structure of control sample are mainly consist of α-helix and random-coil in phosphate-buffered saline (PBS) and phosphate buffered solution with SDS(SDS), the secondary structure of control sample are mainly consist of β-Sheet and random-coil. The CD data also showed that SDS interacts with the gluten protein and modifies the protein conformation, which switched the conformation from α-helix and β-Turn to β-sheet and random-coil. However, the CD analysis also indicated that some of the ordered structures of α-helix, β-Turn and β-sheet were destroyed and converted random-coil coped with acid in two solvents, in other words, the acid treatment can directed change the secondary structure. Furthermore, the effect of comprehensive treatment (DHPM plus acid) is not equal to the simple sum of the individual treatment effect.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 770-773
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.770
• Online since: September 2013
• Authors: Zhao Xi Fang*, Nai Jun Yan, Guo Qin Liu
• Keywords: Different Solvents, Microfluidization, Secondary Structure, Wheat Gluten

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