The Liquid-Solid Phase Transition Characteristic and Protein Binding Property of Composite Cryogel with Embedded Cellulose Beads

Jia Lei Ye

doi:10.4028/www.scientific.net/AMR.781-784.783

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784The Liquid-Solid Phase Transition Characteristic...

The Liquid-Solid Phase Transition Characteristic and Protein Binding Property of Composite Cryogel with Embedded Cellulose Beads

Abstract:

Poly (hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads (pHEMA-CB cryogel) could be of interest as chromatographic medium for bioseparation applications. In this work, liquid-solid phase transition characteristic of the suspension mixture containing HEMA, poly (ethylene glycol) diacrylate (PEGDA), ammonium persulfate (APS), N,N,N',N'-tetramethylethylenediamine (TEMED) and cellulose beads for the production of pHEMA-CB composite cryogel was investigated to reveal the solvent crystallization process occurring in the column. Freezing curves were obtained and the values of initial solvent crystallization temperature T_c and the freezing point temperature T_mc were estimated. Protein binding property of the pHEMA-CB cryogel grafted with (vinylbenzyl) trimethylammonium chloride (VBTAC) was measured and discussed.

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Periodical:

Advanced Materials Research (Volumes 781-784)

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783-786

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.783

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Online since:

September 2013

Authors:

Jia Lei Ye*

Keywords:

Cellulose Bead, Composite Cryogel, Freezing Characteristic, Freezing Curve, Protein

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References

[1] H. Kirsebom and B. Mattiasson: Polym. Chem. Vol. 2 (2011), p.1059.

Google Scholar

[2] F.M. Plieva, H. Kirsebom and B. Mattiasson: J. Sep. Sci. Vol. 34 (2011), p.2164.

Google Scholar

[3] F.M. Plieva, I.Y. Galaev, W. Noppe and B. Mattiasson: Trends Microbiol. Vol. 16 (2008), p.543.

DOI: 10.1016/j.tim.2008.08.005

Google Scholar

[4] F.M. Plieva, I.Y. Galaev and B. Mattiasson: J. Sep. Sci. Vol. 30 (2007), p.1657.

Google Scholar

[5] V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid and B. Mattiasson: Trends Biotechnol. Vol. 21 (2003), p.445.

DOI: 10.1016/j.tibtech.2003.08.002

Google Scholar

[6] X.J. He, K.J. Yao, S.C. Shen and J.X. Yun: Chem. Eng. Sci. Vol. 62 (2007), p.1334.

Google Scholar

[7] J.L. Ye, J.X. Yun, D. -Q. Lin, L.H. Xu, H. Kirsebom, S.C. Shen, G.S. Yang, K.J. Yao, Y. -X. Guan and S. -J. Yao: submitted to J. Chromatogr. A (2013).

Google Scholar

[8] K.J. Yao, J.X. Yun, S.C. Shen and F. Chen: J. Chromatogr. A Vol. 1157 (2007), p.246.

Google Scholar

[9] M.M. Bradford: Anal. Biochem. Vol. 72 (1976), p.248.

Google Scholar

[10] S. Okawa, A. Saito and H. Suto: Int. J. Refrig. Vol. 25 (2002), p.514.

Google Scholar

[11] M. Akyurt, G. Zaki and B. Habeebullah: Energy Convers. Manage. Vol. 43 (2002), p.1773.

Google Scholar