Study on Immobilization of Tannase

Shun Rong He; Xin Ying Wu; Shu Yi Qiu; Xing Huang; Fu Li Li; Ji Ying Feng

doi:10.4028/www.scientific.net/AMR.781-784.774

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Study on Immobilization of Tannase

Study on Immobilization of Tannase

Abstract:

In this paper,tannase was immobilized by the method of the pre cross-linking,embedding and re cross-linking.The orthogonal design is carried out on the basis of single factor,by which the optimum conditions of the immobilized tannase was selected out ,the optimum pre cross-linking and re cross-linking concentration of glutaraldehyde is 0.3%(v/v )and 0.4%(v/v),respectively,the pre cross-linking temperature is 30°C and the re cross-linking time is 1.0h.in addition,the optimum catalytic temperature is 50°C and the optimum catalytic pH is 5.0,and compered with the free tannase ,the temperature and the pH stability of immobilized tannase improved markedly.

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Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

774-778

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.774

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Online since:

September 2013

Authors:

Shun Rong He*, Xin Ying Wu, Shu Yi Qiu, Xing Huang, Fu Li Li, Ji Ying Feng

Keywords:

Cross-Linking, Glutaraldehyde, Immobilisation, Tannase

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