Rapid Determination of Amygdalin and its Novel Degradation Product in Plasma of Mice by SPE-HPLC

Abstract:

Amygdalin was catalytic degraded by extracellular enzymes mixture from Aspergillus niger and a novel product, phenyl-(3,4,5-trihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-acetonitrile (PTMT), with high antitumor activity was identified and purified. To measured the Pharmacokinetic of amygdalin and its products, a simple and rapid SPE-HPLC method was set up. The analysis was performed on an Agilent HPLC system with a C18 ODS column (250 × 4.6 mm i.d.) by gradient elution with 0.05% formic acid in water and 0.05% formic acid in acetonitrile as the gradient mixtures. The flow rate was 1ml/min, the detection wavelength was 215 nm and the column temperature was kept at 25°C. The HPLC assay was carried out within 9 min. The retention times of amygdalin, mandelonitrile, prunasin, PTMT andbenzaldehyde were 3.14, 5.93, 6.82, 7.35 and 8.33 min, respectively. The mean absolute recoveries of three analysts were over 98%. The limit of detection and limit of quantification for were 0.02 and 0.04 μg/ml for amygdalin and benzaldehyde, 0.03 and 0.05 μg/ml for mandelonitrile, and 0.03 and 0.04 μg/ml for prunasin and PTMT.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 83-88
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.83
• Online since: September 2013
• Authors: Chen Xi Wang, Yu Ping Li, Jun Chang*, Bin Zhou, Xiao Han, Jiang Tao Wu, Li Zhen Sun
• Keywords: Amygdalin, Anit-Tumor, Benzaldehyde, Mandelonitrile, phenyl-(3,4,5-Trihydroxy-6-Methyl-Tetrahydro-Pyran-2-Yloxy)-Acetonitrile, Prunasin

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