Construction of Yeast Strain Capable of Co-Fermenting Pentose and Hexose by Protoplast Fusion

Jin Ling Guo; Da Chun Gong; Zhi Jun Li; Zhou Zheng

doi:10.4028/www.scientific.net/AMR.781-784.847

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Construction of Yeast Strain Capable of...

Construction of Yeast Strain Capable of Co-Fermenting Pentose and Hexose by Protoplast Fusion

Abstract:

Saccharomyces cerevisiae R40 and Pachysolen tannophilus P01 were used as the parental strain to construct an engineering strain capable of co-fermenting pentose and hexose by protoplast fusion. A fusant F202 was obtained through inactivating parental protoplasts, screening with YPX solid medium and high glucose liquid medium, ethanol production capacity detecting and identification with PCR-SSR technique. Subsequently, the fermentation performance and genetic stability of F202 was studied. The maximum ethanol production capacity from glucose was 1.47 ml/100 ml with a sugar and alcohol conversion rate 47% which was 11% higher than the parental strain P01. By fermenting xylose the ethanol concentration could achieve to 0.58 ml/100 ml with a sugar and alcohol conversion rate 12%. An ethanol concentration of 1.2 ml/100 ml was obtained by fermenting the mixture of xylose and glucose (mass ratio 1:2). Moreover, no decrease in ethanol yield after 8 generations propagation suggested fustant 202 possessed good genetic stability.

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Periodical:

Advanced Materials Research (Volumes 781-784)

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847-851

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.847

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Online since:

September 2013

Authors:

Jin Ling Guo, Da Chun Gong, Zhi Jun Li, Zhou Zheng

Keywords:

Bioethanol, Biparental Inactivation, Co-Fermenting, Protoplast Fusion, Yeast

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