Effect of Culture Conditions on Production of Xylanase by Trichoderma reesei

Abstract:

Fermentation of xylanase produced by Trichoderma reesei was conducted in 250mL shake flasks. Several fermentation conditions was investigated that affected production of xylanase by Trichod erma reesei, including inoculum age, inoculum size, medium volume, shear stress, temperature, shaking speed and initial pH. Results showed that the the optimum culture conditions were as follows: inoculum age 45h, inoculum size 10%,medium volume 50mL/250mL, shaking speed 200rpm and initial pH4.0(natural). The maximum xylanase activity under each condition was occurred at 104h and the maximal activities can reach 1425.27U/mL.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 856-860
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.856
• Online since: September 2013
• Authors: Rui Huang, Chao Zhang, Hui Tian, Ru Ming Zhao, Fa Shun Yu, Juan Yao, Da Chun Gong*
• Keywords: Culture Condition, Fermentation, Trichoderma reesei, Xylanase

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