Data Mining of Nicotine Demethylase Genes in Modern Tobacoo

Abstract:

The predominant pyridine alkaloid in modern tobacco (Nicotiana tabacum) is Nicotine. However, with the existence of a class of Nicotine demethylases, a large proportion of Nicotine will be converted into Nornicotine during leaf senescence. Identification of Nicotine demethylases in modern tobacco is important, since Nornicotine will be nitrosated into N'-nitrosonornicotine (NNN), which exhibit carcinogenic properties in laboratory animals and is harmful for human heath. Although many efforts have been performed to identified, no comprehensive analysis was carried out for the subfamily of Nicotine demethylases. In this work, we performed a phylogenetic and comparative analysis for Nicotine demethylases with their highly homologs. A single and conserved mutation of W->C will prohibit the activity of Nicotine demethylases. Thus, the proportion of Nornicotine could be dramatically decreased if all Nicotine demethylase were single mutated by transgenic manipulation. Our results provide an insightful and solid foundation for further experimental design.

• Periodical: Advanced Materials Research (Volume 787)
• Pages: 356-360
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.787.356
• Online since: September 2013
• Authors: Jie Lian, De Ji Wang, Jun Ming Xie
• Keywords: Data Mining (DM), Nicotine Demethylase Genes, Tobacco

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