The Role of Beclin1 Gene in Autophagy and Apoptosis Induced by Ionizing Radiation in MCF - 7 Cells

Abstract:

Objective To discuss the role of Beclin1 gene in autophagy and apoptosis induced by ionizing radiation in MCF - 7 cells. Methods MTT assay was used to detect the influence of cells proliferation when beclin-1 gene was over expression and interference. Flow cytometric analysis detected the change of MCF - 7 cells apoptosis after irradiating by X-ray. Western blot method detected total protein changes of beclin - 1. Results Beclin-1 gene was interferred partly, the amount of protein expression of MCF-7-beclin1Ri cells reached to the lowest at 4 h, rose at 8 h, got to the most at 16 h and decreased at 32 h, but still held at higher level;Beclin-1 gene over-expression, the amount of protein expression increased gradually with the extension of time, and got to peak at 32 h. Conclusions Ionizing radiation can stimulate beclin-1 and exist relations of dose - effects in MCF-7 cells.

• Periodical: Advanced Materials Research (Volume 790)
• Pages: 587-589
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.790.587
• Online since: September 2013
• Authors: Ya Li Qi, Yan Jun Liu, Da Li Zhao
• Keywords: Apoptosis, Autophagy, Beclin1, Ionizing Radiation

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