Effect of Chelidonine on the Expressions of Cdk1 and CyclinB1 Proteins in SGC-7901 Cells

Abstract:

To study the effect of chelidonine on Cdk1, p-Cdk1( Thr14), cyclinB1 protein expression in SGC-7901 Cells, and to explore the mechanism of chelidonine inducing G₂/M phase arrest in SGC-7901 Cells. Western Blotting was used to study the effects of chelidonine on the Cdk1, p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells. Chelidonine could significantly decrease the expression of Cdk1 and cyclinB1 protein. Meanwhile, chelidonine could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner. Chelidonine can down-regulate the expression of Cdk1, cyclinB1 and up-regulate p-Cdk1 (Thr14) protein expression in SGC-7901 cells,which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 cells which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 Cells.

• Periodical: Advanced Materials Research (Volume 790)
• Pages: 594-597
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.790.594
• Online since: September 2013
• Authors: Zhong Yuan Qu, Xiang Zou, Jing Wen Zhao, Yu Bin Ji
• Keywords: Cdk1, Chelidonine, CyclinB1, Human Gastric Carcinoma SGC-7901 Cells, p-Cdk(Thr14)

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