Reusable Electrochemical Aptasensor for Sensitive Detection of Small Molecules Based on Structure-Switching Hairpin Probe

Abstract:

A reusable electrochemical biosensing strategy based on structure-switching hairpin probe for the detection of small molecules is proposed using cocaine as the model analyte. Aptamer probe hybridized with the immobilized signal probe to form DNA duplex. When target small molecule was added, competition between target molecule and the signal probe with the aptamer probe happened, which induced the signal probe from stretched duplex to hairpin structure. By measuring ac current voltammetry, the target molecule can be sensitively detected in a linear dynamic range from 1 nM-1000 nM with a low detection limit of 0.7 nM. In particular, the biosensor can be easily regenerated by melting in hot water, making it reusable.

• Periodical: Advanced Materials Research (Volumes 791-793)
• Pages: 988-991
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.791-793.988
• Online since: September 2013
• Authors: Song Bai Zhang, Pei Zhen Han, Ping Lu, Xia Hu, Li Ying Zheng, Xue Wen Liu, Guang Yu Shen, Ji Lin Lu, Li Ping Qiu, Shi Biao Zhou
• Keywords: Aptasensor, Cocaine, Electrochemical, Small Molecule, Structure-Switching

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