Characterization of Putative a (1,3)-β-D-glucan (curdlan) Synthase for a Low Molecular Weight Curdlan Biosynthesis from Agrobacterium sp. M503

Yu Mei Li; Qiang Li; Sheng Han; Dong Xue Song; Yan Hong Qu; Ting Ting Liu; Yu Xia Zheng; Ying Zi Liu; Zhi Wen Zhao

doi:10.4028/www.scientific.net/AMR.807-809.2031

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 807-809Characterization of Putative a (1,3)-β-D-glucan...

Characterization of Putative a (1,3)-β-D-glucan (curdlan) Synthase for a Low Molecular Weight Curdlan Biosynthesis from Agrobacterium sp. M503

Abstract:

A β-(1,3)-D-glucan (curdlan) synthase gene for a low molecular weight curdlan biosynthesis, crdS_Ag, from Agrobacterium sp. M503 was cloned and its encoding protein was characterized by several online protein analysis softwares. The crdS_Ag consists of 1965-base-pairs Open Reading Frame (ORF) encoding a protein with molecular weight approximate 73.5 kDa, which contains the conserved domain of CESA-CelA_like belonging to glycosyltransferase family 2 (GT2). Moreover, CrdS_Ag was a membrane protein with seven hydrophobic transmembrance domains. The second structure analysis indicated it was composed of 43.12% α-helix, 17.89% β-sheet, and 38.99% random coil structure. These data will lay a foundation to clarify the biosynthesis mechanism of the low molecular weight curdlan.

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Periodical:

Advanced Materials Research (Volumes 807-809)

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2031-2034

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.807-809.2031

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Online since:

September 2013

Authors:

Yu Mei Li, Qiang Li, Sheng Han, Dong Xue Song, Yan Hong Qu, Ting Ting Liu, Yu Xia Zheng, Ying Zi Liu, Zhi Wen Zhao

Keywords:

Curdlansynthase, Low Molecular Weight, β-(1 3)-D-Glucan

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