Microbiological Distribution of Multi-Level-well Samples in the Coast of South China

Abstract:

In this study, the Microbiological communities wereinvestigated by culture-independent molecular approaches. Four 16S rRNA genelibraries from mineral waterand different groundwater of wells collected from Zhuhai of the South China were constructed and analyzed by amplifiedribosomal DNA restriction analysis technique (ARDRA). The clones of dominantARDRA patterns were selected to sequence. The phylogenetic analysis indicatedthat the sequences from dominant ARDRA patterns belonged to Proteobacteria, Bacteroidetes，archaea ,Actinobacteria,Planctomycetes, and Candidate division OP3, OP8, OP11 et al. Thepercentages of Proteobacteria isolated from multi-level-well samples D1,D5,D6were respectively 32.3％,34.3%,46.7%;The Bacteroidetes were respectively 5.0％,11.2％,6.5％; and Actinobacteria were respectively 5.4％,6.6％,7.8％. As a background,the seawater clone library, with the majority (32.8%) of the clones clusteringwithin the Proteobacteria, the second most abundant group were Bacteroidetesand Planctomycetes(both 9.3%), The results suggest that microbialcommunity from different vertical layers ofgroundwater in pristine habitats that have been described for the first time inChina.

• Periodical: Advanced Materials Research (Volumes 807-809)
• Pages: 380-384
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.807-809.380
• Online since: September 2013
• Authors: Xiao Lan Huang, Jian Yao Chen, Ge Ying Lai
• Keywords: Distribution, Microbial Community, Multi-Level-Well, Pristine Habitats

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