Degradation Behaviour of Thin Polymer Films of Poly(Amide Ester) Hydrogel Using Quartz Crystal Microbalance

Abstract:

Quartz Crystal Microbalance (QCM) has been typically used as a mass sensor in the study of monolayer and multilayer depositions and dissolution, mass transport in polymer films on electrodes, corrosion processes at electrodes and mass changes caused by protein adsorption at electrodes. Thin films of an aromatic poly (amide ester) hydrogel with hydrolyzable cross links were prepared in-situ on a gold-coated quartz crystal which is the transducing element of a Quartz Crystal Microbalance (QCM). Here we report the synthesis and degradation behavior of an aromatic poly (amide ester) hydrogel with hydrolyzable cross links using QCM. Degradation of the hydrogel films was found to proceed with Δf increment, indicating mass decrease and is in line with the theory proposed by Sauerbrey. Films with a higher cross-link density underwent partial degradation with swelling; and are depicted as a decrease in the Δf values with time. Deviation from the general base catalysis of ester hydrolysis was observed at higher base concentration due to the biphasic environment of the hydrolysis reaction.

• Periodical: Advanced Materials Research (Volume 812)
• Pages: 38-45
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.812.38
• Online since: September 2013
• Authors: Dzaraini Kamarun, Ahmat Norizan, Steffi Krause, Chris Hunter, Lilia Milanesi
• Keywords: Crosslink Density, Degradation, Hydrogel, Quartz Crystal Microbalance

References

[1] X. Zhao and J. M. Harris, "Novel Degradable Poly (Ethylene Glycol) Hydrogels for Controlled Release of Protein." Journal of Pharmaceutical Sciences 87(11): (1998)1450-1458.

DOI: 10.1021/js980065o

[2] J. L. Wes, and J. A. Hubbell, "Polymeric Biomaterials with Degradation Sites for Proteases Involved in Cell Migration." Macromolecules 32: (1999) 241-241.

DOI: 10.1021/ma981296k

[3] B. K.Mann, A. S.Gobin, A. T.Tsai, R. H. Schmedlen and J. L. West "Smooth Muscle Cell Growth in Photo polymerized Hydrogels with Cell Adhesive and Proteolytically Degradable Domains: Synthetic ECM Analogs for Tissue Engineering." Biomaterials 22(22): (2001) 3045-3051.

DOI: 10.1016/s0142-9612(01)00051-5

[4] S. Kim and K.E. Healy, "Synthesis and Characterization of Injectable Poly (N-Isopropylacrylamide-Co-Acrylic Acid) Hydrogels with Proteolytically Degradable Cross-Links." Biomacromolecules 4(5): (2003) 1214-1223.

DOI: 10.1021/bm0340467

[5] S.Kim, E. H.Chung, M. Gilbert and K. E. Healy, "Synthetic MMP-13 Degradable ECMs Based on Poly (N-Isopropylacrylamide-Co-Acrylic Acid) Semi-Interpenetrating Polymer Networks. I. Degradation and Cell Migration." J.of Biomedical Materials Research.75A(1): (2005) 73-88.

DOI: 10.1002/jbm.a.30375

[6] M. E. Bryne, K.Park and N. A. Peppas, "Molecular Imprinting within Hydrogels." Advanced Drug Delivery Reviews 54: (2002) 149-161.

DOI: 10.1016/s0169-409x(01)00246-0

[7] D. Kamarun, X.W. Zheng, L. Milanesi, C.A. Hunter, S. Krause, "A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase." Electrochimica Acta 54 (22): (2009) 4985 – 4990.

DOI: 10.1016/j.electacta.2009.03.067

[8] C. A. Hunter, P. S. Jones, P. Tiger and S. Tomas, "Chemical Triple-Mutant Boxes for Quantifying Cooperativity in Intermolecular Interactions." Chemistry - A European Journal 8(23): (2002) 5435-5446.

DOI: 10.1002/1521-3765(20021202)8:23<5435::aid-chem5435>3.0.co;2-v

[9] A. Sabot, and S.Krause, "Simultaneous Quartz Crystal Microbalance Impedance and Electrochemical Impedance Measurements: Investigation into the Degradation of Thin Polymer Films." Analytical Chemistry 74(14): (2002) 304-3311.

DOI: 10.1021/ac0200724

[10] M.E. Alf, T.A. Hatton, K.K. Gleason "Initiated chemical vapor deposition of responsive polymeric surfaces." Thin Solid Films 519: (2011) 4412–4414.

DOI: 10.1016/j.tsf.2011.01.286

[11] A. Valentina , B. Monica B, Z. Antonella , Z. Alessandra, P. Elisabetta, M. Lucia, R. Ross Fluoropolymers coatings on polydimethylsiloxane for retarding swelling in toluene." Thin Solid Films 520 (2012) 2293–2300

DOI: 10.1016/j.tsf.2011.09.063

[12] G. Z. Sauerbrey, "Use of Quartz Vibrator for Weighing Thin Films on a Microbalance." Zeitschrift Fur Physik 155: (1959) 206-210.

[13] P. L. Konash and G. J. Bastiaans, Piezoelectric Crystals as Detectors in Liquid Chromatography." Analytical Chemistry 52: (1980) 1929-1931.

DOI: 10.1021/ac50062a033

[14] V. E. Bottom, Introduction to Quartz Crystal Unit Design. New York, Van Nostrand Reinhold Co. (1982).

[15] M. A. M. Noel and , P. A. Topart "High-Frequency Impedance Analysis of Quartz Crystal Microbalances. 1.General Considerations." Analytical Chemistry 66: (1994) 484-491.

DOI: 10.1021/ac00076a011