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Biomediated Separation of Kaolinite and Hematite Using Bacillus subtilis

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Abstract:

Bacillus subtilis was used to demonstrate microbially induced selective flocculation to separate kaolinite and hematite. In neutral pH range of 7 - 8, 90 - 95% of hematite was selectively flocculated whereas 80 - 85% of kaolinite was dispersed using hematite - grown cells. Hematite- grown cells exhibited significant adsorption onto hematite than onto kaolinite, compared to unadapted cells. Kaolinite grown Bacillus subtilis secreted significant amounts of mineral specific proteins which conferred surface hydrophobicity whereas hematite-grown cells secreted more polysaccharides rendering hematite hydrophilic. Bacterial extracellular protein (EP) was isolated and the protein profiles of bacteria grown in the absence and presence of minerals were established.

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Periodical:

Advanced Materials Research (Volume 825)

Pages:

223-226

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.825.223

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Online since:

October 2013

Authors:

S. Poorni, K.A. Natarajan

Keywords:

Bacillus subtilis, Biobeneficiation, Flocculation, Hematite, Kaolinite

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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