A Novel Apparatus to Determine the Bio-Oxidation Kinetics of Sessile Leptospirillum ferriphilum

Abstract:

A novel apparatus was developed to test the ferrous iron oxidation kinetics of Leptospirillum ferriphilum in predominantly sessile culture, by combining a CSTR under wash-out conditions with a packed bed of inert ceramic saddles. Results indicate that a dense culture of sessile bacteria is established rapidly, which achieves high oxidation rates in all experiments with a yield in terms of CO₂ uptake rates comparable or higher to what has been measured in planktonic culture. However, the yield in terms of cell counts changes dramatically, indicating a substantial shift in carbon utilisation in the sessile culture. The apparatus is feasible as a method to study iron oxidation kinetics of sessile cultures, providing it is operated at sufficiently high recycle ratios.

• Periodical: Advanced Materials Research (Volume 825)
• Pages: 238-241
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.825.238
• Online since: October 2013
• Authors: Porogo Duku, Sanet H. Minnaar, Susan T.L. Harrison, Jochen Petersen
• Keywords: Bio-Oxidation, Kinetic, Leptospirillum ferriphilum, Planktonic, Residence Time, Sessile, Wash-Out

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