Investigation of Microbial Cellulose/Cotton/Silver Nanobiocomposite as a Modern Wound Dressing

Abstract:

In this study the nanobiocomposite of Microbial cellulose/Cotton/Silver is introduced as a modern wound dressing. Microbial cellulose was synthesized in situ on cotton gauze. The static medium culture (Hestrin & Scharm) and Acetobacter Xylinum used for microbial cellulose synthesis and 6 days formed layers used for experiments. Half of 6 days samples were reverse after 3 days in order to form double coated gauze. One or two coated sides specimens were deactivated purified and dipped in a 500ppm nanosilver concentration. Specimens were analyzed by X-ray diffraction method, Fourier transform infra-red spectroscopy, Scanning electron microscopy and transmission electron microscopy. Their water and moisture absorption determined and their antibacterial efficiency evaluated by AATCC 100 antibacterial test method. Results showed about 30% increase in water absorption with less than 8% moisture regain. Microscopic images showed a proper distribution of nanosilver without agglomerations at surface and inside nanobiocomposite which caused improved antimicrobial efficiency. The obtained results indicated that nanocomposite (double coated gauze) has high potential for applying as a modern wound dressing.

• Periodical: Advanced Materials Research (Volume 829)
• Pages: 616-621
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.829.616
• Online since: November 2013
• Authors: Ramin Khajavi*, Amin Meftahi, Somayeh Alibakhshi, Leila Samih
• Keywords: Acetobacter Xylinum, Microbial Cellulose/Cotton/Nanosilver, Modern Wound Dressing, Nanobiocomposite

References

[1] R. Czajka, Development of Medical Textile Market, Fibers Text. East. Eur. 13 (2005) 13-15.

[2] S.K. Chinta, K.V. Veena, Impact of Textiles in Medical Field, Int. J. of Latest Trends in Eng. and Tech. 2 (2013) 142-145.

[3] Petersen, Nathan, P. Gatenholm, Bacterial cellulose-based materials and medical devices: current state and perspectives, Appl. Microbiol. Biotechnol., 91 (2011) 1277-1286.

DOI: 10.1007/s00253-011-3432-y

[4] WK. Czaja , DJ. Young , M. Kawecki , RM Jr. Brown . The future prospects of microbial cellulose in biomedical applications, BioMacromol. 8. 1 (2007): 1-12.

[5] C. Zhijiang, J. Kim, Bacterial cellulose/poly (ethylene glycol) composite: characterization and first evaluation of biocompatibility, Cellul. 17. 1 (2010) 83-91.

DOI: 10.1007/s10570-009-9362-5

[6] L. Nimeskern, J. Sundberg, P. Gatenholm, R. Muller,  K. S. Stok Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement, J. Mech. behavior of biome. Mater. (2013).

DOI: 10.1016/j.jmbbm.2013.03.005

[7] B. M. Cherian, Alcides Lopes Leão, S. F. de Souza, G. M. de Olyveira, L. M. Manzine Costa, C. V. Seullner Brandão, S. Narine, Bacterial Nanocellulose for Medical Implants, Adv. Nat. Polym., Springer Berlin Heidelberg, (2013) 337-359.

DOI: 10.1007/978-3-642-20940-6_10

[8] M. Ul-Islam, T. Khan, W. A. Khattak, J. K. Park, Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties, Cellul. (2013) 1-8.

DOI: 10.1007/s10570-012-9849-3

[9] Z. Cai, H. Chengwei, Y. Guang, Poly (3-hydroxubutyrate-co-4-hydroxubutyrate)/bacterial cellulose composite porous scaffold: Preparation, characterization and biocompatibility evaluation, Carbohydr. Polym. 87. 2 (2012) 1073-1080.

DOI: 10.1016/j.carbpol.2011.08.037

[10] G. Miguel, P. Gatenholm, D. Klemm, Bacterial Cellulose: A Sophisticated Multifunctional Material, CRC Press, 9 (2012).

[11] F. G Torres, O. P. Troncoso, C. Torres, C. J. Grande, Cellulose Based Blends, Composites and Nanocomposites, Adv. Nat. Polym., Springer Berlin Heidelberg, (2013) 21-54.

DOI: 10.1007/978-3-642-20940-6_2

[12] W. Ch. Lin, Ch. Lien, H. J. Yeh, Ch. M. Yu, Sh. H. Hsu  Lin, Bacterial Cellulose and Bacterial Cellulose-Chitosan Membranes for Wound Dressing Applications, Carbohydr. Polym. (2013).

DOI: 10.1016/j.carbpol.2013.01.076

[13] S. Saska, L. N. Teixeira, P. Tambasco de Oliveira, A. M. Minarelli Gaspar,  S. JoséLima Ribeiro, Y. Messaddeqa, R. Marchettoa, Bacterial cellulose-collagen nanocomposite for bone tissue engineering, J. Mater. Chem. 22. 41 (2012) 22102-22112.

DOI: 10.1039/c2jm33762b

[14] P. Chen, J. Lai, H. Hsiao, Y. Chu, Ch. Liao, U.S. Patent 20, 120, 308, 649 (2012).

[15] Belgacem, M. Naceur, and A. Gandini, eds. Monomers, polymers and composites from renewable resources, Access Online via Elsevier, (2011).

[16] A. Meftahi, R. Khajavi, A. Rashidi, M. Sattari, M. E. Yazdanshenas, M. Torabi, The effects of cotton gauze coating with microbial cellulose, Cellul. 17. 1 (2010)199-204.

DOI: 10.1007/s10570-009-9377-y