Bioactivity Assay of Chitosan Microspheres Containing Pig Thymosin In Vitro

Pei Ni; Rong Rong Zhan; Yu Long Wei; Jing Hui; Feng Qing Hu

doi:10.4028/www.scientific.net/AMR.830.455

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 830Bioactivity Assay of Chitosan Microspheres...

Bioactivity Assay of Chitosan Microspheres Containing Pig Thymosin In Vitro

Abstract:

Thymosin, composed of many peptides, is distributed in animal and has immunomodulating effects. However, many factors could affect its bioactivity. Chitosan microspheres containing pig thymosin (CMPT) were prepared through W/O emulsion cross-linking method. In this study, thymosin activity between pig-thymosin and CMPT was investigated through E-rosette test and mouse spleen lymphocyte proliferation (MPLP) experiment in vitro. Results showed that CMPT has the same immunomodulatory activity as pig thymosin. When pig-thymosin was 1.5 μg / ml, E-rosette binding rate was 63%, and E-rosette binding rate of CMPT reached 60%。MPLP rate was 73.3% and 68.5%, respectively. CMPT have the capacity to keep the E-rosette binding and MPLP activity of pig thymosin. This may help to improve traditional formulation and bioavailability of thymosin, and expand its scope of application in clinic.

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Advanced Materials Research (Volume 830)

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455-458

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https://doi.org/10.4028/www.scientific.net/AMR.830.455

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October 2013

Authors:

Pei Ni, Rong Rong Zhan, Yu Long Wei, Jing Hui, Feng Qing Hu

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Bioactivity Assay, Chitosan Microspherescontaining Pig Thymosin

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