Optimization of the Heterologous Expression of Sesuvium portulacastrum Betaine Aldehyde Dehydrogenase in Escherichia coli

Cheng Long Yang; Rui Mei Li; Yang Zhou; Rui Jun Duan; Shao Ping Fu; Jiao Liu; Xin Wen Hu; Jian Chun Guo

doi:10.4028/www.scientific.net/AMR.864-867.221

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Optimization of the Heterologous Expression of...

Optimization of the Heterologous Expression of Sesuvium portulacastrum Betaine Aldehyde Dehydrogenase in Escherichia coli

Abstract:

A full-length sequence coding for a betaine aldehyde dehydrogenase gene from S. portulacastrum was cloned into expression vector pGEX-4T-1, and named pGEX-4T-SpBADH. The GST-SpBADH fusion protein was expressed and the expression conditions were optimized. Through the research on optimization of expression the concentration of IPTG, concentration of bacterium, induction time and temperature and so on, the results showed, the expression of GST-SpBADH increased accompany with the induction time. The expression level of GST-SpBADH fusion protein reached the highest for 5 h cultured and for OD₆₀₀ is about 0.6 at 37°C, 0.2 mmol/L IPTG can effectively induce the expression of GST-SpBADH in Escherichia coli expression system.

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Periodical:

Advanced Materials Research (Volumes 864-867)

Pages:

221-224

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.864-867.221

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Online since:

December 2013

Authors:

Cheng Long Yang, Rui Mei Li, Yang Zhou, Rui Jun Duan, Shao Ping Fu, Jiao Liu, Xin Wen Hu, Jian Chun Guo*

Keywords:

Betaine Aldehyde Dehydrogenase, Prokaryotic Expression, S. portulacastrum

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References

[1] Lokhande V.H., Nikam T.D., Patade V.Y. and Suprasanna P. Morphological and molecular diversity analysis among the Indian clones of Sesuvium portulacastrum L. Genet. Resour. Crop Evol., 2009, 56: 705-717.

DOI: 10.1007/s10722-008-9396-9

Google Scholar

[2] Lokhande V.H., Nikam T.D. and Suprasanna P. In vitro culture, plant regeneration and clonal behaviour of Sesuvium portulacastrum L.: a prospective halophyte. Physiol. Mol. Biol. Plants, 2010, 16: 187-193.

DOI: 10.1007/s12298-010-0020-z

Google Scholar

[3] M.B. Burg and J. D. Ferraris. Intracellular organic osmolytes: function and regulation , J. Biol. Chem., 2008, 283: 7309-7313.

DOI: 10.1074/jbc.r700042200

Google Scholar

[4] Trossat C., B. Rathinasabapathi and A.D. Hanson. Transgenically expressed betaine aldehyde dehydrogenase efficiently catalyzes oxidation of dimethylsulfoniopropionaldehyde and omega-aminoaldehydes. Plant Physiol. (Rockville), 1997, 113: 1457–1461.

DOI: 10.1104/pp.113.4.1457

Google Scholar

[5] Livingstone J.R., Maruo T., Yoshida I., Tarui Y., Hirooka K., Yamamoto Y., Tsutui N. and Hirasawa E. Purification and properties of betaine aldehyde dehydrogenase from Avena sativa. J. Plant Res., 2003, 116: 133–140.

DOI: 10.1007/s10265-003-0077-7

Google Scholar

[6] Hongzi Cong, Xiyan Yu, Xiufeng Wang and Qinghua Shi. Cloning and Expression Analyzing of One BADH Gene CmBADH of Muskmelon Under Abiotic Stress. Acta Horticulturae Sinica, 2013, 40(5): 905–912.

Google Scholar

[7] V. Vasiliou, A. Bairoch, K.F. Tipton and D.W. Nebert. Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping, Pharmacogenetics, 1999, 9: 421-434.

DOI: 10.1097/fpc.0b013e3283329023

Google Scholar

[8] D. Rhodes and A.D. Hanson. Quaternary ammonium and tertiary sulfonium compounds in higher plants. Annu. Rev. Plant Physiol. Mol. Biol., 1993, 44: 357-384.

DOI: 10.1146/annurev.pp.44.060193.002041

Google Scholar