Genotoxicity of 1-1-Butyl-3-Methylimidazolium Ionic Liquids on Pakchoi (Brassica campestris L. ssp. Chinensis) Using ISSR Markers

Abstract:

Ionic liquids (ILs) are one class of important industrial chemicals that may replace conventional organic solvents. Due to the solubility, the toxicity study of ILs focused on aquatic mediums. However, there is seldom research for the the toxicity on terrestrial organism. In this paper, we studied the toxicity of three imidazolium ionic liquids 1-butyl-3-methyl imidazolium chloride ([Bmim][CL]), 1-butyl-3-methyl imidazolium tetrafluoroborate ([Bmim][BF₄]), and 1-butyl-3-methyl imidazolium trifluoroacetate ([Bmim][CF₃COOH]) on seed germination and seedling growth of pakchoi. Inter-simple sequence repeat (ISSR) marker technique was applied to detect DNA damage induced by imidazolium ILs. The results showed that all of three ILs could inhibit seed germination and seedling growth of pakchoi significantly at higher concentration (≥16 mM/L). At same concentration (1 mM/L and 4 mM/L), the genotoxicity of three ILs was following the trends: [Bmim][BF₄]> [Bmim][CF₃COOH]> [Bmim][CL]. It was concluded that ISSR assay offered a useful way to detect toxicity of ILs contamination on plant at low concentration even without transformation of physiological feature, such as germination and growth parameters and so on.

• Periodical: Advanced Materials Research (Volumes 864-867)
• Pages: 3-10
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.864-867.3
• Online since: December 2013
• Authors: Kun Zeng, Yong Lai Xue, Xia Hong, Fang Ma, Chang Ri Han, Dao Lin Du*
• Keywords: Genotoxicity, Imidazolium Ionic Liquids, ISSR, Pakchoi

References

[1] D.S.H. Wong, J.P. Chen, J.M. Chang and C.H. Chou: Equilibria Vol. 194-197 (2002), p.1089.

[2] J. Ranke, K. Molter, F. Stock, U. Bottin-Weber, J. Poczobutt, J. Hoffmann, B. Ondruschka, J. Filser and B. Jastorff: Ecotoxicol. Environ. Saf. Vol. 58 (2004), p.396.

DOI: 10.1016/j.ecoenv.2004.09.004

[3] C. Samori, A. Pasteris, P. Galletti and E. Tagliavini: Environ. Toxicol. Chem. Vol. 26 (2007), p.2379.

[4] R.J. Bernot, E.E. Kennedy and G.A. Lamberti: Environ. Toxicol. Chem. Vol. 24 (2005), p.1759.

[5] C.W. Cho , T.P.T. Pham , Y.C. Jeon and Y.S. Yun: Green Chem. Vol. 10 (2008), p.67.

[6] C. Pretti, C. Chiappe, I. Baldetti, S. Brunini, G. Monni and L. Intorre: Ecotoxicol. Environ. Saf. Vol. 72 (2009), p.1170.

DOI: 10.1016/j.ecoenv.2008.09.010

[7] K.J. Angelis, M. McGuffie, M. Menke and I. Schubert: Environ. Mol. Mutagen. Vol. 36 (2000), p.146.

[8] B. Ateeq, M. Abul Farah and W. Ahmad: Ecotoxicol. Environ. Saf. Vol. 62 (2005), p.348.

[9] M. Gonzalez, S. Soloneski, M.A. Reigosa and M.L. Larramendy: Toxicol. In Vitro Vol. 19 (2005), p.289.

[10] K.C. Sorensen, J.W. Stucki, R.E. Warner, E.D. Wagner and M.J. Plewa: Environ. Mol. Mutagen. Vol. 46 (2005), p.174.

[11] C. Pretti, C. Chiappe, D. Pieraccini, M. Gregori, F. Abramo, G. Monnia and L. Intorrec: Green Chem. Vol. 8 (2006), p.238.

[12] S.H. Wang, P.P. Huang, X.Y. Li, C.Y. Wang, W.H. Zhang and J.J. Wang: Environ. Toxicol. Vol. 25 (2010), p.243.

[13] Y.R. Luo, X.Y. Li, X.X. Chen, B.J. Zhang, Z.J. Sun and J.J. Wang: Environ. Toxicol. Vol. 23 (2008), p.736.

[14] S.P. Ventura, A.M. Goncalves, F. Goncalves and J.A. Coutinho: Aquat. Toxicol. Vol. 96 (2010), p.290.

[15] L.S. Wang, L. Wang, L. Wang, G. Wang, Z.H. Li and J. J. Wang: Environ. Toxicol. Vol. 24 (2009), p.296.

[16] W. Liu, Y.S. Yang, P.J. Li, Q.X. Zhou, L.J. Xie and Y.P. Han: J Hazard. Mater. Vol. 161 (2009), p.878.

[17] A. Mengoni, C. Gonnelli, F. Galardi, R. Gabbrielli and M. Bazzicalupo: Mol. Ecol. Vol. 9 (2000), p.1319.

[18] S. Ünyayar, A. Çelik, FÖ Çekiç and A. Gözel: Mutagenesis Vol. 21 (2006), p.77.

[19] A. Latala, M. Nedzi and P. Stepnowski: Green Chem. Vol. 11 (2009), p.580.

[20] A. Latala, M. Nedzi and P. Stepnowski: Green Chem. Vol. 12 (2010), p.60.

[21] K.M. Docherty, S.Z. Hebbeler and Jr C.F. Kulpa: Green Chem. Vol. 8 (2006), p.560.

[22] Z. Zhang, J.F. Liu, X.Q. Cai, W.W. Jiang, W.R. Luo and G.B. Jiang: Environ. Sci. Technol. Vol. 45 (2011), pp.1688-94.

[23] E. Zietkiewicz, A. Rafalski and D. Labuda: Genomics Vol. 20 (1994), p.176.

[24] M. Pradeep Reddy, N. Sarla and E.A. Siddiq: Euphytica Vol. 128 (2002), p.9.

[25] S.P. Joshi, V.S. Gupta, R.K. Aggarwal, P.K. Ranjekar and D.S. Brar: Theor. Appl. Genet. Vol. 100 (2000), p.1311.

[26] F. Al-Qurainy: Afr. J. Biotechnol. Vol. 9 (2010), p.467.

[27] T. Gichner, Z. Patkova, J. Szakova and K. Demnerova: Mutat. Res. Vol. 559 (2004), p.49.

[28] J.J. Doyle and J.L. Doyle: Focus Vol. 12 (1990), p.13.

[29] J.G. Williams, A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey: Nucleic. Acids. Res. Vol. 18 (1990), p.6531.

DOI: 10.1093/nar/18.22.6531

[30] R. Herrera, V. Cares, M.J. Wilkinson and P.D.S. Caligari: Euphytica Vol. 124 (2002), p.139.

DOI: 10.1023/a:1015693721532

[31] F.A. Atienzar, B. Cordi, M.E. Donkin, A.J. Evenden, A.N. Jha and M.H. Depledge: Aquat. Toxicol. Vol. 50 (2000), p.1.

[32] C. Luceri, C. De Filippo, G. Caderni, L. Gambacciani, M. Salvadori, A. Giannini and P. Dolara: Carcinogenesis Vol. 21 (2000), p.1753.

DOI: 10.1093/carcin/21.9.1753

[33] P. Balczewski, B. Bachowska, T. Bialas, R. Biczak, W.M. Wieczorek and A. Balinska: J. Agric. Food Chem. Vol. 55 (2007), p.1881.

[34] S. Studzinska and B. Buszewski: Anal. Bioanal. Chem. Vol. 393 (2009), p.983.

[35] B. Liu and J.F. Wendel: Mol. Ecol. Notes Vol. 1 (2001), p.205.

[36] F.A. Atienzar and A.N. Jha: Mutat. Res. Vol. 613 (2006), p.76.

[37] W. Liu, P.J. Li, X.M. Qi, Q.X. Zhou, L. Zheng, T.H. Sun and Y.S. Yang: Chemosphere Vol. 61 (2005), p.158.

[38] A. Castaño and C. Becerril: Mutat. Res-Fond. Mol. M. Vol. 552 (2004), p.141.

[39] S. Cenkci, İ.H. Ciğerci, M. Yıldız, C. Özay, A. Bozdağ and H. Terzi: Environ. Exp. Bot. Vol. 67 (2010), p.467.

DOI: 10.1016/j.envexpbot.2009.10.001

[40] H. Wang, S.V. Malhotra and A.J. Francis: Chemosphere Vol. 82 (2011), p.1597.

[41] A.C. Skladanowski, P. Stepnowski, K. Kleszczynski and B. Dmochowska: Environ. Toxicol. Pharmacol. Vol. 19 (2005), p.291.