The Influence of the Peptide Molar Ratios on the Functionalization of Gold Nanoparticles

Abstract:

Au nanoparticles (NPs) functionalized with L-cysteine (Cys) and cysteine-glycine (Cys-Gly) were synthetized. The AuNPs were prepared using sodium citrate as reducing agent. The influence of the molar concentrations of Cys and Cys-Gly, as well as the sodium citrate is studied on particle size and particle size distribution. TEM measurements revealed the formation of AuNPs with diameter in the range 5-35 nm which corresponds to nontoxic sizes [we should add a reference here, perhaps number one]. The optimal particle size for biomedical application along with narrow particle size distribution was observed for samples prepared with molar ratio of CAu:Ccitrate = 1:10. The results of UV-Vis spectroscopy revealed the interaction of the AuNPs with Cys and Gly-Cys demonstrated by a visible change in the absorption intensities of the plasmon peak located at 520 nm after AuNP functionalization and a slight shifting of this gold nanoparticles plasmon peak. Thus, any dielectric shell on surface of particles with more refraction index (and, correspondingly, dielectric function) can produce the particles with the red shift. Such effect of the surface shell with red-shift in the range of few nanometers observed for the AuNPs functionalized with Cys and Cys-Gly (Fig. 4) can be interpreted as thin or discontinuous layer of aminoacid molecules according to the data of optical spectra simulation.

• Periodical: Advanced Materials Research (Volume 872)
• Pages: 94-105
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.872.94
• Online since: December 2013
• Authors: Mayra C. Ramirez-Camacho, Inga Tuzovskaya, Nina Bogdanchikova, Alexey Pestryakov, Arturo Susarrey-Arce, Valerii Gurin
• Keywords: Cysteine, Functionalization, Glycine, Gold Nanoparticle, Sodium Citrate

References

[1] S. Krol et al., Nano Lett., 6 (9) (2006) 1933–(1939).

[2] T.H.L. Nghiem1, T.H. La, X.H. Vu, et. al, Adv. Nat. Sci.: Nanosci. Nanotechnol. 1 025009 (2010) 5.

[3] V.A. Sinani, D. S. Koktysh,B. -G. Yun, R.L. Matts, T.C. Pappas, M. Motamedi, S.N. Thomas, N.A. Kotov, Nano Lett. 3 (9) (2003) 1177-1182.

DOI: 10.1021/nl0255045

[4] Y. Zhang, N. Kohler, M. Zhang, Biomaterials 23 (2002) 1553–1561.

[5] B. Kang, M. A. Mackey, M. A. El-Sayed, J. Am. Chem. Soc., 132 (2010), 1517–1519.

[6] P.C. Chen, S.C. Mwakwari, A.K. Oyelere, Nanotechnology, Science and Applications, 1 (2008) 45-66.

[7] E. E. Connor, J. Mwamuka, A. Gole, C. J. Murphy, M. D. Wyatt, Small, 1 (2005) 325–327.

[8] I. Willner, E. Katz, B. Willner, R. Blonder, V. Heleg-Shabtai and A.F. Bückmann, Biosens. Bioelectron., 12 (1997) 337–356.

DOI: 10.1016/s0956-5663(96)00065-6

[9] P. Tengvall, M. Lestelius, B. Liedberg and I. Lundstroem, Langmuir, 8 (1992) 1236–1238.

DOI: 10.1021/la00041a001

[10] H. -A. Weng, C. -C. Wu, C. -C. Chen, C. -C. Ho, S. -J. Ding, Journal of Materials Science: Materials in Medicine, 21 (5) (2010) 1511-1519.

[11] C. M. Goodman, C. D. McCusker, T. Yilmaz, V. M. Rotello, Bioconjugate Chem., 15 (2004) 897–900.

[12] A. Mocanu, I. Cernica, G. Tomoaia, L. -D. Bobos, O. Horovitz, M. Tomoaia-Cotisel, Colloids and Surfaces A: Physicochem. Eng. Aspects, 338 (2009) 93–101.

DOI: 10.1016/j.colsurfa.2008.12.041

[13] G. Sonavane, K. Tomoda, K. Makino, Colloids Surf. B, 66 (2008) 274–280.

[14] B.J. Spencer, I. M. Verma, Proc. Natl. Acad. Sci. USA, 104 (18) (2007) 7594–7599.

[15] J. Turkevich; P. C. Stevenson, J. Hillier; Discuss. Faraday Soc., 11 (1951) 55.

[16] T.B. Huff, M.N. Hansen, Y. Zhao, J.X. Cheng, A. Wei, Langmuir, 23 (2007) 1596–1599.

[17] T. Niidome, M. Yamagata, Y. Okamoto, Y. Akiyama, H. Takahashi, T. Kawano, Y. Katayama, Y. Niidome, J. Controlled Release, 114 (2006) 343–347.

DOI: 10.1016/j.jconrel.2006.06.017

[18] K.B. Male, J. Li, C.C. Bun, S. -C. Ng, J.H.T. Luong, J. Phys. Chem. C, 112 (2) (2008) 443–451.

[19] H. Takahashi, Y. Niidome, T. Niidome, K. Kaneko, H. Kawasaki, S. Yamada, Langmuir, 22 (2006), 2–5.

DOI: 10.1021/la0520029

[20] G. Sonavanea, K. Tomodaa, K. Makino, Colloids and Surfaces B: Biointerfaces 66 (2008) 274–280.

[21] S. Link and M. A. El-Sayed , Phys. Chem. B, 103 (1999) 4212-4217.

[22] R. Le´vy, N. T. K. Thanh, R. C. Doty, I. Hussain, R. J. Nichols, D. J. Schiffrin, M. Brust, and D. G. Fernig J. AM. CHEM. SOC., 126 (2004) 10076-10084.

DOI: 10.1021/ja0487269

[23] A. Majzik, R. Patakfalvi, V. Hornok and I. Dekany, Gold Bulletin Volume 42 No 2 (2009).

[24] T.H.L. Nghiem, T.H. La, X. H. Vu, V.H. Chu, T.H. Nguyen, et. al Adv. Nat. Sci.: Nanosci. Nanotechnol. 1 (2010) 025009.

[25] Yu. Petrov, Clusters and small particles, Moscow, Nauka (1982) 298.

[26] G. Mie, Ann. Phys. (Leipzig) 25, 377 (1908). U. Kreibig, M. Vollmer, Optical Properties of Metal Clusters. Berlin: Springer-Verlag (1995).

[27] V. Petranovskii, V. Gurin, N. Bogdanchikova, A. Licea-Claverie, Y. Sugi, E. Stoyanov, Mater. Sci. Eng. A332 (2002) 180.

[28] D.L. Feldheim, C.A. Foss, Jr, Metal Nanoparticles: Synthesis, Characterization and Applications, Marcel Dekker, New York (2002) 141.

[29] Yu. Ekmanis, Yu. Rud, I. Radchenko, J. Phys. Techn. Sci., 5 (1997) 3.

[30] P.B. Johnson, R.W. Christy, Phys. Rev. B, 6 (1972) 4370.

[31] Y.Q. He, L. Kong, Z.F. Liu, Spectrochemica Acta Part A, , 61, (2005) 2861.

[32] L. Marsich, A. Bonifacio, S. Mandal, S. Krol, C. Beleites, and V. Sergo Langmuir 2012, 28, 13166.

DOI: 10.1021/la302383r