Optimization of HS-SPME for GC-MS Analysis of Volatiles Compounds in Roasted Sesame Oil

Abstract:

This paper was to develop a simple and rapid headspace solid-phase microextraction (HS-SPME) method coupled with gas chromatography–mass spectrometry (GC-MS) for the determination of volatiles compounds from the roasted sesame oil (RSO). A HP-5MS capillary column (30 m × 0.25 mm I.D. × 0.25 mm film thick) was used for GC-MS, and a 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used to extract volatiles compounds. The condition was optimized by varying the sample-to-headspace ratio (0.5-2.5 g/15 ml), extraction time (10-50 min) and Splitless time (0.5-4 min). The results showed that the optimal operating conditions occurred at (extraction temperature:40°C, sample-to-headspace ratio: 1.5 g/15 ml, extraction time: 40 min, Splitless time: 1 min) for the analyze method.

• Periodical: Advanced Materials Research (Volumes 881-883)
• Pages: 61-64
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.881-883.61
• Online since: January 2014
• Authors: Zhao Xi Fang, Guo Qin Liu*, Xue De Wang, Li Juan Han, Bing Ge Liu
• Keywords: DVB-CAR-PDMS Fibre, Headspace SPME-GC-MS, Roasted Sesame Oil

References

[1] Lee, S. W., Jeung, M. K., Park, M. H., Lee, S. Y., & Lee, J… Effects of roasting conditions of sesame seeds on the oxidative stability of pressed oil during thermal oxidation. Food chemistry, 118(3) (2010), 681-685.

DOI: 10.1016/j.foodchem.2009.05.040

[2] Garcia, R., Martins, N., & Cabrita, M. J… Putative Markers of Adulteration of Extra Virgin Olive Oil with Refined Olive Oil: Prospects and Limitations. Food Research International (2013).

DOI: 10.1016/j.foodres.2013.05.008

[3] García-González, D. L., & Aparicio, R… Research in olive oil: challenges for the near future. Journal of agricultural and food chemistry, 58(24) (2010), 12569-12577.

DOI: 10.1021/jf102735n

[4] Zhang, J., Li, L., Gao, N., Wang, D., Gao, Q., & Jiang, S… Feature extraction and selection from volatile compounds for analytical classification of Chinese red wines from different varieties. Analytica chimica acta, 662(2) (2010), 137-142.

DOI: 10.1016/j.aca.2009.12.043

[5] Mildner-Szkudlarz, S., & Jeleń, H. H.. The potential of different techniques for volatile compounds analysis coupled with PCA for the detection of the adulteration of olive oil with hazelnut oil. Food Chemistry, 110(3) (2008), 751-761.

DOI: 10.1016/j.foodchem.2008.02.053

[6] Lee, E., & Choe, E.. Changes in oxidation-derived off-flavor compounds of roasted sesame oil during accelerated storage in the dark. Biocatalysis and Agricultural Biotechnology, 1(1) (2012), 89-93.

DOI: 10.1016/j.bcab.2011.08.003

[7] Cecchi, T., & Alfei, B.. Volatile Profiles of Italian Monovarietal Extra Virgin Olive Oils via HS-SPME-GC-MS: Newly Identified Compounds, Flavors Molecular Markers, and Terpenic profile. Food Chemistry (2013).

DOI: 10.1016/j.foodchem.2013.05.090

[8] Kalua, C. M., Bedgood Jr, D. R., & Prenzler, P. D.. Development of a headspace solid phase microextraction-gas chromatography method for monitoring volatile compounds in extended time–course experiments of olive oil. Analytica chimica acta, 556(2) (2006).

DOI: 10.1016/j.aca.2005.09.050

[9] Contini, M., & Esti, M.. Effect of the matrix volatile composition in the headspace solid-phase microextraction analysis of extra virgin olive oil. Food chemistry, 94(1) (2006), 143-150.

DOI: 10.1016/j.foodchem.2004.12.039

[10] Panseri, S., Soncin, S., Chiesa, L. M., & Biondi, P. A.. A headspace solid-phase microextraction gas-chromatographic mass-spectrometric method (HS-SPME–GC/MS) to quantify hexanal in butter during storage as marker of lipid oxidation. Food chemistry, 127(2) (2011).

DOI: 10.1016/j.foodchem.2010.12.150

[11] Keszler, Á., & Héberger, K… Influence of extraction parameters and medium on efficiency of solid-phase microextraction sampling in analysis of aliphatic aldehydes. Journal of Chromatography A, 845(1), (1999) 337-347.

DOI: 10.1016/s0021-9673(99)00332-5

[12] Pawliszyn, J. (Ed. )… Applications of solid phase microextraction (Vol. 5). Royal Society of Chemistry(1999).