Determination of Picogram Levels of Levodopa in Pharmaceutical Preparations and Biofluids by Flow-Injection Chemiluminescence

Abstract:

An ultrasensitive method for determination of L-dopa at picogram levels by flow-injection chemiluminescence (FI-CL) as presented for the first time, based on the quenching effect of levodopa (L-dopa) on the luminol-lysozyme reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of L-dopa concentration ranging from 3.0 to 7.0 × 10³ pg mL^-1 (R = 0.9967), with the limit of detection (LOD) of 1.0 pg mL^-1 (3σ). The proposed procedure was successfully applied to the determination of L-dopa in pharmaceutical preparations, human saliva, serum, and urine samples with the recoveries ranging from 96.7% to 104.3% and RSDs less than 4.0% (n = 5).

• Periodical: Advanced Materials Research (Volumes 884-885)
• Pages: 566-569
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.884-885.566
• Online since: January 2014
• Authors: Yun Zhang*, Su Ya Gao
• Keywords: Chemiluminescence, Flow Injection, Levodopa, Luminol, Lysozyme

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