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Using 2D-PAGE and Mass Spectrometry (LC-MS/MS) for Embryo Proteins Analysis in Antheraea pernyi

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Abstract:

To study the dynamic variation of protein components and contents in Antheraea pernyi, 2D-PAGE, in this job, was used to investigate embryo development. Some special protein spots expression profiles changed obviously were analyzed by mass spectrometry. 2D-PAGE results showed that the protein spots increased from 370 at the development stage of 12 h to 422 at 300 h. Eighteen special protein spots were identified by LC-MS/MS, and 8 kinds of proteins are found to include heat shock proteins families, Vitellogenin, KK-42-binding protein, actin-depolymerizing factor 1, etc., which are mainly involved in some important processes. Analyze the function of those proteins, so as to accumulate basic information on embryonic development in A. pernyi.

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Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

556-559

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.884-885.556

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Online since:

January 2014

Authors:

Yong Wang, Yi Ren Jiang, Li Qin*

Keywords:

2D-Electrophoresis, Antheraea pernyi, Embryo Development, Mass Spectrometry

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] Y. Zhang, Z.Y. Qiu, Q.L. Zhao: submitted to Science of Sericulture (2010).

Google Scholar

[2] I. Minoru, K. Hidenori, K. Takuya: submitted to ChemBioChem (2006).

Google Scholar

[3] Y. Wang, Y.Z. Li, B.Y. Wang: submitted to Science of Sericulture (2011).

Google Scholar

[4] G. Candiano, M. Bruschi, L. Musante: submitted to Electrophoresis (2004).

Google Scholar

[5] J.H. Postlethwait, F. Giorgi: Vitellogenesis in insects (Developmental Biology, New York 1985).

Google Scholar

[6] M.X. Lu, X.Y. Teng, C.S. Yang: submitted to Science of Sericulture (1990).

Google Scholar

[7] T. Shimizu, T. Shiotsuki, A. Seino: submitted to Journal of Insect Biotechnology and Sericology (2002).

Google Scholar

[8] Y.P. Li, R.X. Xia, H. Wang: submitted to International Journal of Biological Sciences (2009).

Google Scholar

[9] B. Li, Q.Y. Xia, H. Fujii: submitted to Journal of Agricultural Biotechnology (2005).

Google Scholar

[10] S.P. Tammariello, L.D. David: submitted to Insect Biochemistry and Molecular Biology (1998).

Google Scholar

[11] X.N. Zhao, F.D. Tong, C.Y. Jiang: submitted to Bulletin of Sericulture (2009).

Google Scholar

[12] X.L. Tong, F.Y. Dai, S.Y. Ma: submitted to Insect Molecular Biology (2008).

Google Scholar

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