The Effects of Floating Granulosa Cells Density on the Developmental Competence of Bovine Oocytes: A Study Using the Well-in-Well Culture System

Wei Guo Feng; Chun E Ren; Zhi Fang Pan

doi:10.4028/www.scientific.net/AMR.884-885.544

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 884-885The Effects of Floating Granulosa Cells Density on...

The Effects of Floating Granulosa Cells Density on the Developmental Competence of Bovine Oocytes: A Study Using the Well-in-Well Culture System

Abstract:

In this study, we succeed to establish the well in the well (WIW) culture system. Using this system we studied the effect of floating granulosa cells density on oocyte developmental competence. Results: Apoptosis rate of the floating cells were high (73.576.3%) and did not differ (P > 0.05) among follicles of different sizes. Hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WIW system (13.3%) and those cultured in the group system (16.3%). In 4-8mm follicles, oocyte developmental competence is higher (P < 0.05) in follicles with 1030×10⁵/mL floating cells than in follicles with < 10 or >30%×10⁵/mL floating cells.

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Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

544-547

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.884-885.544

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Online since:

January 2014

Authors:

Wei Guo Feng, Chun E Ren, Zhi Fang Pan*

Keywords:

Density, Developmental Competence, Floating Granulosa Cell, Oocyte

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References

[1] Bilodeau-Goeseels S, Panich P. Effects of oocyte quality on development and transcriptional activity in early bovine embryos. Anim Reprod Sci 2002; 71: 143–55.

DOI: 10.1016/s0378-4320(01)00188-9

Google Scholar

[2] Vajta G, Peura TT, Holm P, Paldi A, Greve T, Trounson AO, et al. New method for culture of zona-included or zona-free embryos: the well of the well (WOW) system. Mol Reprod Dev 2000; 55: 256–64.

DOI: 10.1002/(sici)1098-2795(200003)55:3<256::aid-mrd3>3.0.co;2-7

Google Scholar

[3] Palasz AT, Thundathil J. The effect of volume of culture medium and embryo density on in vitro development of bovine embryos. Theriogenology 1998; 49: 212.

DOI: 10.1016/s0093-691x(98)90565-9

Google Scholar

[4] The effects of granulosa cell apoptosis on the developmental competence of bovine oocytes: A study using the well-in-well culture system. International Conference on Complex Medical Engineering, 2013; 5: 504-7.

DOI: 10.1109/iccme.2013.6548301

Google Scholar

[5] Duvall E, Wyllie AH. Death and the cell. Immunol Today1986; 7: 115–8.

Google Scholar

[6] Yang MY, Rajamahendran R. Morphological and biochemical identiﬁcation of apoptosis in small, medium, and large bovine follicles and the effects of follicle-stimulating hormone and insulin-like growth factor-I on spontaneous apoptosis in cultured bovine GCs. Biol Reprod 2000; 62: 1209–17.

DOI: 10.1095/biolreprod62.5.1209

Google Scholar