Synthesis of Hydroxyapatite Nanoparticle from Tutut (Bellamya javanica) Shells by Using Precipitation Method for Artificial Bone Engineering

Abstract:

The synthesis and characterization of both structural and microstructure of hydroxyapatite (HAp) nanoparticles by using precipitation method have been performed. Orthophosphoric acid solution (H₃PO₄) 0.3 M was dropped into the suspension of Ca(OH)₂ from tutut (Bellamya javanica) shells at pH 10 and added by NH₄OH 1 M at the final deposition process. The precipitate was filtered and then heated in a furnace at temperature of 1000 °C for 3 hours. The result of elemental analysis showed that the Ca/P ratio of HAp sample was 1.71. X-ray diffraction patterns showed that the sample was a single phase of Ca₁₀(PO₄)₆(OH)₂ with hexagonal structure (P63/m) and lattice parameters of a = b = 9.418(3) Å, c = 6.885(2) Å, a = b = 90^o, g = 120^o, V = 528.9(4) ų, and r = 3.084 g cm^-3. The as-synthesized HAp has crystallite and particle sizes of 16.1055 and 89.15 nm, respectively. The TEM image revealed that the HAp particles have spherical-polygonal shape with an average diameter particles of around 10-20 nm. It was concluded that the synthesis of HAp nanoparticles from tutut (Bellamya javanica) shells by using a wet precipitation method has been successfully carried out and the as-synthesized HAp was potential to be applied as materials for artificial bone engineering.

• Periodical: Advanced Materials Research (Volume 896)
• Pages: 284-287
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.896.284
• Online since: February 2014
• Authors: Lenita Herawaty*, Eti Rohaeti, Charlena Charlena, Sulistioso Giat Sukaryo
• Keywords: Crystal Structure, Hydroxyapatite (HA), Nanoparticle, Precipitation, Tutut (Bellamya javanica) Shells

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