Plasmids Role in Survival of Acinetobacter calcoaceticus A1 Exposed to UV-Radiation and Hydrocarbons

Abstract:

The role of plasmids in hydrocarbon-degrading bacteria Acinetobacter calcoaceticus A1 survival to UV-radiation and hydrocarbons was studied. Natural plasmids-containing A. calcoaceticus A1 showed high resistance to UV-radiation. A. calcoaceticus A1 showed active growth under exposed to UV-radiation for up to 30 minutes. Combined effects of UV-radiation and petroleum hydrocarbons did not considerably reduce the growth of strains. It was shown a stimulating effect of UV-radiation on the growth curves of strains of A. calcoaceticus A1. Constructed recombinant strain (E.coli XL blue Rec) showed the ability to grow on medium with addition petroleum hydrocarbons. Combined effects of UV-radiation and petroleum hydrocarbons have had a negative effect on the growth of E.coli XL blue Rec. Thus, results showed that the plasmid DNA of natural hydrocarbon-degrading bacteria A. calcoaceticus A1 may contain genes of microbial resistance to UV - radiation and petroleum hydrocarbons.

• Periodical: Advanced Materials Research (Volume 905)
• Pages: 151-155
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.905.151
• Online since: April 2014
• Authors: Assemgul Bekturova*, Zhannur Markhametova, Zhaksylyk Masalimov
• Keywords: Acinetobacter calcoaceticus A1, E.coli XL Blue Rec, Plasmids, UVC - Radiation

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