Selection of a Mutant Strain Streptococcus equi SH-109 and Optimization of Culture Conditions for the Production of High Molecular Weight Hyaluronic Acid

Jian Cheng Feng; Rong Hu Zhang

doi:10.4028/www.scientific.net/AMR.915-916.909

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 915-916Selection of a Mutant Strain Streptococcus equi...

Selection of a Mutant Strain Streptococcus equi SH-109 and Optimization of Culture Conditions for the Production of High Molecular Weight Hyaluronic Acid

Abstract:

A high molecular weight hyaluronic acid-producing mutant, designated as Streptococcus equi SH-109 that did not produce streptolysin or hyaluronidase was selected from wild-type Streptococcus equi SH-0 by NTG treatment. The selected mutant could produce high molecular weight HA and had perfect genetic stability after reproduction. Several culture conditions were optimized in a 5-l fermentor for the production of hyaluronic acid. It was found that the optimal pH and agitation speed were 7.6 and 550rpm, respectively. Under the optimized culture condition, hyaluronic acid with a molecular weight of 4.0 x 10⁶ was accumulated in the culture broth.

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Periodical:

Advanced Materials Research (Volumes 915-916)

Pages:

909-912

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.915-916.909

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Online since:

April 2014

Authors:

Jian Cheng Feng, Rong Hu Zhang*

Keywords:

Culture Conditions, High Molecular Weight, Hyaluronic Acid, Streptococcus equi

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