Advanced Materials Research Vol. 933

Abstract: Under the experimental conditions, , specific PCR can can quickly and accurately separate the Streptococcus thermophilus from three categories of dairy products, 14.kinds of dairy products,Both primers CR1 and CR2 optimal annealing temperature was 58.0 °C, annealing temperature of CR3 is 50.0 °C, and those temperatures were used to ensure that the primer CR1, CR2 and CR3 with CRISPR sequences effective annealing, no other non-specific reaction. Gram-positive purple chain coccus, there are differences in morphology, from different samples .The CTAB method was used to extract DNA. After PCR amplification, obtaining 11 different CRISPR sequences amplification results. In six samples of yogurt, there are five kinds of CRISPR sequences, four milk samples obtain three kinds of CRISPR sequences, three kinds of cheese has three deferent CRISPR sequences.

Abstract: Denim is one important clothing product recently in the market. However, the original solid blue colour is not attractive to the consumers and hence many manufacturers are trying to remove part of the blue colour from the denim fabric surface. This fading effect could impart fade-look or worn out effect in the final products which made it becomes popular to the consumers. There are different methods to achieve the colour fading effect in which enzyme washing is one of the simplest methods to be used in the industry. Although the enzyme washing is a good method for colour fading of denim, the process involves the use of chemical as well as temperature which may introduce certain degree of damage in denim fabric. Thus, this paper will investigate the effect of enzyme washing on the tensile property of denim fabric under different treatment duration.

Abstract: The dyeing effects of atmospheric pressure impregnation method on thick Birch and Elm veneer under bleached and unbleached treatment were discussed in this paper, as well as the color changes and influencing factors analyzed by CIE(1976)L^*a^*b^*color system. The results showed that it was feasible to dye the thick Birch veneer using red acid GR under unbleached treatment and both the color saturation and gloss met the requirements while disperse dyes were not suitable for the thick veneer because of the general results, poor penetration, and low uptake rate. The dyeing effect on Elm was not satisfactory so that it was not recommended as dyeing wood while its better material quality made it better for furniture.

Abstract: In this research, the primary study in extracting technologies of Tea polyphends was finished. On the basis of the water lixiviation by orthogonal optimization method, extraction process of TP by microwave extraction was optimized. The best combined condition of the water lixiviation as follows: liquid-lolid ratio 25:1, extraction temperature 95°C , extraction time 20min,Under this conditions, the yield of TP is 13.993 %.And the best combined condition by microwave extraction: liquid-lolid ratio 35:1, microwave power 750 W, extraction time 18min, and the yield of TP is 20.3023 % through duplicated experiment. The results showed that compare with water lixiviation, the yield of TP was enhanced by 6.3093 % by microwave method. Meanwhile, the extraction time by microwave method was reduced greatly.

Abstract: With the development of high temperature protective material, thermal barrier coatings are used in the field of aircraft and industrial steam turbine. They have characteristics of complex structure and long-term work in high temperature environment, etc. Because of multilayer material physical properties mismatch, oxidation, interface roughness, creep stress and so on, the coatings system is easy to produce cracks, expand and link near the interface of coating during thermal cycling. By establishing I, II, III, IV four numerical models of crack fracture mechanisms, assuming that the thickness of oxidation layer is constant, considering the influence of interface roughness and material properties mismatch in the process of thermal cycling, it gets the results of residual stress field near the crack and the influence of the interface. Compared to the presence of defects, four kinds of crack failure mechanisms are analyzed. Energy release rate is calculated by the virtual crack closure method, which can conclude the cracks of I, IV tip stress concentration phenomena are obvious, crack II is easy to expand, due to the temperature load, crack III does not appear stress concentration phenomenon at the tip.

Abstract: The inhibition effect of medium density fiberboard (MDF), particle board, dyed veneer and poplar veneer photochromic coated with water-borne wood paint and wax oil were studied by CIE(1976)L^*a^*b^* color measurement system. The results showed that the specimen surface towards yellowing, lightness reduced, yellow-blue chromatic index rose and color saturation increased. Coating treatment has good inhibition effect on woodiness material photochromic mainly depends on it can significantly inhibit the yellow-blue index increase.

Abstract: Selective Laser Sintering/Selective Laser Melting (SLS/SLM) is one of Additive Manufacturing (AM) processes that utilize layer by layer powder deposition technique and successive laser beam irradiation based on Computer Aided Design (CAD) data. During laser irradiation on metal powders, melt pool was formed, which then solidified to consolidated structure. Therefore, melt pool is an important behavior that affects the final quality of track formation. The study investigates the melt pool behavior through visualization of the consolidation process during the single track formation on the first layer. In order to understand the transformation process of metal powder to consolidated structure and mechanism involved, high speed camera was used to monitor the process. Yb:fiber laser beam was irradiated on metal powder at maximum power of 150W. The laser processing parameters such as laser power, scan speed and layer thickness were varied in order to investigate their influence on the consolidation process. The result shows the size of melt pool increased with laser power and decreasing with increment in scan speed. Furthermore, with the increase of layer thickness, melt pool formation was unstable with chaotic movement. Significant amount of molten powder splattering was recorded from the melt pool. At high layer thickness also, the molten powder formed spherical shaped and the solidified molten powder failed to wet with the substrate.

Abstract: Hydraulic fracturing was first used in the late 1940s and has become a common technique to enhance the production of low-permeability formations.Hydraulic fracturing treatments were pumped into permeable formations with permeable fluids. This means that as the fracturing fluid was being pumped into the formation, a certain proportion of this fluid will being lost into formation as fluid leak-off. Therefore, leak-off coefficient is the most leading parameters of fracturing fluids. The accurate understanding of leak-off coefficient of fracturing fluid is an important guidance to hydraulic fracturing industry design. In this paper, a new field method of leak-off coefficient real time analysis model was presented based on instantaneous shut-in pressure (ISIP). More than 100 wells were fractured using this method in oil field. The results show that average liquid rates of post-fracturing was 22m³/d which double improvement compared with the past treatment wells. It had an important role for hydraulic fracturing stimulation treatment design in low permeability reservoirs and was proven that the new model for hydraulic fracturing treatment is greatly improved.

Abstract: In the course of systematic modeling, the artificial neural networks method is studied. In allusion to the defect of grads descension of traditional back propagation network algorithms, some improving measures have been taken to determine the optimal prediction and analysis model. These measures include adaptive learning, additive momentum, reasonable selection of drive function, and using genetic algorithm to optimize the input parameters. And to learn and predict the utilization of blast furnace production data, better application result is acquired.

Abstract: Based on Pam-Stamp software platform, standardization procedure of finite element analysis for sheet metal forming was customized by set up module such as model building, meshing, setting of boundary conditions, calculation submitting, viewing of results and report generating. Standardization procedure has been successfully applied in the development of new products, which shortens the preparation cycle of procedure, improves the forming quality of the parts and enhances the capability for rapidly researching and developing.