Vacuum Assisted Extraction of Indirubin from Radix isatidis through Orthogonal Test Method

Abstract:

A new vacuum assisted extraction (VAE) method was established and applied for the extraction of indirubin from Radix Isatidis. The major factors of VAE process such as boiling temperature, ethanol level, extraction time and extraction cycles, which have influence on the extraction yield of indirubin were tested by an orthogonal array L₉ (3⁴).The results show that the optimum extraction conditions are ethanol level 40% (v/v), boiling temperature 60 °C, extraction time 3 h and extraction 3 cycles. Under these conditions the extraction efficiency of indirubin reaches 1.091 mg/g, which is 30% higher than that by conventional heat reflux extraction at atmospheric pressure. The present results demonstrated that VAE is an efficient, simple and fast method for extracting indirubin from Radix Isatidis, which shows great potential for becoming an alternative technique for industrial scale-up applications.

• Periodical: Advanced Materials Research (Volumes 941-944)
• Pages: 1174-1177
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.941-944.1174
• Online since: June 2014
• Authors: Ya Qi Wang, Zhen Feng Wu, Ji Ping Lan, Ming Yang*
• Keywords: Indirubin, Orthogonal Experiment, Radix isatidis, Vacuum Assisted Extraction

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