The Expression Analysis of the YABBY Transcript Factor-SlYABBY2a in the Tomato Seedling under Low Light

Abstract:

YABBY family proteins are plant-specific transcriptional factors which have been identified to play roles in the growth and development of plants. The expression character of YABBY genes was important to gene functions. Members of the YABBY gene have been cloned in tomato. Our results showed that SlYABBY2 was expressed in stems, leaves and stem apexes, but barely in roots of MLK1(Lycopersicon esculentum Mill) and FL1(Lycopersicon esculentum Mill) tomato. Meanwhile, the effect of low light on SlYABBY2 expression was analyzed in MLK1 and FL1. The results showed that SlYABBY2 expression decreased after low light in leaves and stem apex of MLK1 and FL1 tomato. All results suggested that SlYABBY2 might be transcribed in stems, leaves and stem apexes and low light might have an effect on SlYABBY2 expression.

• Periodical: Advanced Materials Research (Volumes 941-944)
• Pages: 1163-1167
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.941-944.1163
• Online since: June 2014
• Authors: Qing Bo Zhang, Ying Liu, Hui Li, Tian Lai Li*
• Keywords: Gene Expression, Low Light, Tomato, YABBY Genes

References

[1] Klee, H. J. and Giovannoni, J. J: Genetics and control of tomato fruit ripening and quality attributes. Annu Rev Genet. 45: 41–59(2011).

DOI: 10.1146/annurev-genet-110410-132507

[2] Kimura, S. and Sinha, N: Tomato (Solanum lycopersicum): a model fruit-bearing crop. CSH Protoc. 10. 1101/pdb. emo105(2008).

DOI: 10.1101/pdb.emo105

[3] Sun, J. Q., Jiang, H.L. and Li, C. Y: Systemin/jasmonate-mediated systemic defense signaling in tomato. Mol Plant. 4: 607–615(2011).

DOI: 10.1093/mp/ssr008

[4] Rajani, S. and Pia, G. S: Differentiating Arabidopsis Shoots from Leaves by Combined YABBY Activities. The Plant Cell. 22: 2113-2130(2010).

DOI: 10.1105/tpc.110.075853

[5] Simon, M.K., Williams, L.A., Brady-Passerini K., Brown, R.H. and Gasser, C. S: Positive- and negative-acting regulatory elements contribute to the tissue-specific expression of INNER NO OUTER, a YABBY-type transcription factor gene in Arabidopsis. BMC Plant Biol. 12: 214(2012).

DOI: 10.1186/1471-2229-12-214

[6] Juarez, M. T., Twigg, R. W. and Timmermans, M. C. P: Specification of adaxial cell fate during maize leaf development. Development. 131: 4533–4544(2004).

DOI: 10.1242/dev.01328

[7] Zhao, W., Su, H. Y., Song, J., Zhao, X. Y. and Zhang, X. S: Ectopic expression of TaYAB1, a member of YABBY gene family in wheat, causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis. Plant Science. 170: 364–371(2006).

DOI: 10.1016/j.plantsci.2005.09.008

[8] Toriba, T., Harada, K., Takamura, A., Nakamura, H., Ichikawa, H., Suzaki, T. and Hirano, H. Y: Molecular characterization of the YABBY gene family in Oryza sativa and expression analysis of OsYABBY1. Mol. Genet. Genomics. 277: 457–468(2007).

DOI: 10.1007/s00438-006-0202-0

[9] Xiang, J., Liu, R. Q., Li, T. M., Han, L. J., Zou, Y., Xu, T. F., Wei, J. Y., Wang, Y. J., Xu, Y: Isolation and characterization of two VpYABBY genes from wild Chinese Vitis pseudoreticulata. Protoplasma. 250(6): 1315-25(2013).

DOI: 10.1007/s00709-013-0514-y

[10] Zhang, X. L., Yang, Z. P., Zhang, J. and  Zhang, L. G: Ectopic expression of BraYAB1-702, a member of YABBY gene family in Chinese cabbage, causes leaf curling, inhibition of development of shoot apical meristem and flowering stage delaying in Arabidopsis thaliana. 14(7): 14872-91(2013).

DOI: 10.3390/ijms140714872

[11] Lin, Z. W., Li, X. R. and Shannon, L. M: Parallel domestication of the Shattering1 genes in cereals. Nature genetics. 44(6): 720(2012).

[12] Huang, Z. J., Houten, J. V., Gonzalez, G., Han, X. and Knaap, E. V. D: Genome-wide identification, phylogeny and expression analysis of SUN, OFP and YABBY gene family in tomato. Mol Genet Genomics. 288: 111–129(2013).

DOI: 10.1007/s00438-013-0733-0

[13] Cong, B., Barrero, L. S. and Tanksley, S. D: Regulatory change in YABBY-like transcription factor led to evolution of extreme fruit size during tomato domestication. Nat Genet. 40: 800–804(2008).

DOI: 10.1038/ng.144

[14] Yoshida, S., Mandel, T. and Kuhlemeier, C: Stem cell activation by light guides plant organogenesis. Genes Dev. 25: 1439-1450(2011).

DOI: 10.1101/gad.631211

[15] Bowman, J. L: The YABBY gene family and abaxial cell fate. Plant Biol. 3: 17–22(2000).

DOI: 10.1016/s1369-5266(99)00035-7

[16] Sarojam, R., Sappl, P. G., Goldshmidt, A., Efroni, I., Floyd, S. K., Eshed, Y. and Bowman, J. L: Differentiating Arabidopsis shoots from leaves by combined YABBY activities. Plant Cell. 22: 2113-2130(2010).

DOI: 10.1105/tpc.110.075853

[17] Byrne and Mary, E: Making leaves. Plant Biology. 15: 24–30(2012).

[18] Stahle, M. I., Kuehlich, J., Staron, L., Arnim, A. G., and Golz, J. F: YABBYs and the transcriptional corepressors LEUNIG and LEUNIG HOMOLOG maintain leaf polarity and meristem activity in Arabidopsis. Plant Cell. 21: 3105-3118(2009).

DOI: 10.1105/tpc.109.070458