An New Electrochemical Sensor for DNA Ligase Detection

Abstract:

We herein constructed a sensor that converts T4 DNA ligase-induced conformational transformation of the probe DNA to electrochemical response based on host-guest recognition and nanoparticle label. In the sensor, the hairpin dsDNA terminal-labeled with 4-((4-(dimethylamino)phenyl)azo)benzoic acid (dabcyl) and thiol group was immobilized on Au electrode surface as the probe by Au-S bond, and the CdS nanoparticles surface-modified with β-cyclodextrins (CdS-CD) were employed as electrochemical signal provider and host-guest recognition element. After T4 DNA ligase repairing single-strand breaks in duplex DNA, the dsDNA probe underwent a significant conformational change, which forced dabcyl away from the electrode. As a result, formerly shielded dabcyl became accessible to host-guest recognition between β-cyclodextrin and dabcyl, thus the target repairing event could be sensitively transduced to electrochemical signal provided by CdS-CD.

• Periodical: Advanced Materials Research (Volumes 945-949)
• Pages: 2013-2016
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.945-949.2013
• Online since: June 2014
• Authors: Hao Fan*, Guo Bing Wei, Fu Sheng Liao, Nian Hong
• Keywords: Cyclodextrins, dsDNA, Electrochemical Sensor, Nanoparticle, T4 DNA Ligase

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