Isolation and Characterization of Petroleum Hydrocarbon Degrading Bacteria from the Bohai Sea, China

Abstract:

Fourteen petroleum hydrocarbon degrading bacteria strains were isolated from oil-contaminated site. Isolated strains were able use diesel oil as sole carbon and energy source. Bacterial strain HD1 was selected due to the luxuriant growth on oil agar. The oil degradation rate of strain HD1 was analyzed using UV-spectrometry-based methods. The result showed that the rate of diesel oil degradation of 75% was observed after 14days of cultivation.

• Periodical: Advanced Materials Research (Volumes 955-959)
• Pages: 728-731
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.955-959.728
• Online since: June 2014
• Authors: Ping Guo, Jian Guo Lin*, Bin Xia Cao, Na Ta
• Keywords: Biodegradability, Biodegradation, Oil Contamination

References

[1] I.M. Head, D.M. Jones, and W.F. Roling: Marine microorganisms make a meal of oil. Nat. Rev. Microbiol. Vol. 4 (2006), p.173–182.

DOI: 10.1038/nrmicro1348

[2] R. R. Colwell, J. D. Walker, and J. J. Cooney: Ecological aspects of microbial degradation of petroleum in the marine environment. Critical Reviews in Microbiology, vol. 5, no. 4(1977), p.423–445.

DOI: 10.3109/10408417709102813

[3] D. Nilanjana, and C. Preethy: Microbial Degradation of Petroleum Hydrocarbon Contaminants: An overview. Biotechnology Research International. vol. (2011)，doi：10. 4261/2011/941810.

[4] J. I. Medina-Bellver, P. Mar´ın, and A. Delgado et al：Evidence for in situ crude oil biodegradation after the Prestige oil spill. Environmental Microbiology. vol. 7, no. 6(2005), p.773–779.

DOI: 10.1111/j.1462-2920.2005.00742.x

[5] T. M. April, J. M. Foght, and R. S. Currah: Hydrocarbon degrading filamentous fungi isolated from flare pit soils in northern and western Canada. Canadian Journal of Microbiology. vol. 46, no. 1(2000), p.38–49.

DOI: 10.1139/w99-117

[6] W. Ulrici: Contaminant soil areas, different countries and contaminant monitoring of contaminants, in: Environmental Process II. Soil Decontamination Biotechnology, edited by H. J. Rehm and G. Reed, Eds., vol. 11(2000), p.5–42.

DOI: 10.1002/9783527620951.ch1

[7] J. G. Leahy and R. R. Colwell: Microbial degradation of hydrocarbons in the environment. Microbiological Reviews. vol. 54, no. 3(1990), p.305–315.

DOI: 10.1128/mr.54.3.305-315.1990

[8] R.P.J. Swannell, K. Lee, M. McDonagh: Field evaluations of marine oil spill bioremediation. Microbiological Reviews 60(1996), p.342–365.

DOI: 10.1128/mr.60.2.342-365.1996

[9] L.G. Whyte, J. Hawari, E. Zhou, L. Bourbonnière, W.E. Inniss, and C.W. Greer: Biodegradation of variable-chain-lenght alkanes at low temperatures by a psychrotrophic Rhodococcus sp. Applied and Environmental Microbiology vol. 64(1998).

DOI: 10.1128/aem.64.7.2578-2584.1998

[10] F. Rojo: Degradation of alkanes by bacteria. Environmental Microbiology vol. 11(2009), p.2477–2490.

[11] D.W.S. Westlake, A.M. Jobson and F.D. Cook, 1978. In situ degradation of oil in a soil of the boreal region of the Northwest Territories. Can.J. Microbiol. 24: 254–260.

DOI: 10.1139/m78-044

[12] J.D. Van Hamme, A. Pera, M. Agnolucci, and M.M. Valdrighi, 1997. Humic acids stimulate growth and activity of in vitro tested axenic cultures of soil autotrophic nitrifying bacteria. Biol. Fertil. Soils 24: 243–248.

DOI: 10.1007/s003740050238

[13] R.M. Atlas: Effects of hydrocarbons on micro-organisms and biodegradation in Arctic ecosystems, in: Petroleum Effects in the Arctic Environment, edited by F. R. Engelhardt, Ed., p.63–99, Elsevier, London, UK, (1985).

[14] G. Floodgate: The fate of petroleum in marine ecosystems, in: Petroleum Microbiology, edited by R. M. Atlas, Ed., p.355–398, Macmillion, New York, NY, USA, (1984).

[15] J. C. Okolo, E. N. Amadi, and C. T. I. Odu: Effects of soil treatments containing poultry manure on crude oil degradation in a sandy loam soil. Applied Ecology and Environmental Research, vol. 3, no. 1(2005), p.47–53.

DOI: 10.15666/aeer/0301_047053

[16] K. S. M. Rahman, T. J. Rahman, Y. Kourkoutas, I. Petsas, R. Marchant, and I. M. Banat: Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Bioresource Technology. vol. 90, no. 2(2003).

DOI: 10.1016/s0960-8524(03)00114-7

[17] R. J. W. Brooijmans, M. I. Pastink, and R. J. Siezen,: Hydrocarbon-degrading bacteria: the oil-spill clean-up crew. Microbial Biotechnology, vol. 2, no. 6(2009), p.587–594.

DOI: 10.1111/j.1751-7915.2009.00151.x

[18] M. M. Yakimov, K. N. Timmis, and P. N. Golyshin: Obligate oil-degrading marine bacteria. Current Opinion in Biotechnology, vol. 18, no. 3(2007), p.257–266.

DOI: 10.1016/j.copbio.2007.04.006