This paper presents the results of an experimental investigation of fretting wear characteristics on the contact surface of press-fitted shaft subjected to a cyclic bending load. A series of interrupted fretting wear tests with press-fitted specimens were carried out by using a rotating bending fatigue test machine. The evolution of contact surface profile of press-fitted shaft due to fretting wear were measured with a profilometer. The local wear coefficient during the running-in period is discussed from experimental results and FE analysis. It is found that the maximum depth of fretting wear by repeated slip between shaft and boss occurred at the close of contact edge at the early stage of fatigue life and the regions of worn surface are expanded to the inner side of contact edge as increasing number of fatigue cycles. The initial fretting wear rate at the early stage of fatigue life increased rapidly at all loading condition. After steep increasing, the increase of wear rate is nearly constant in the low bending load condition. The local wear coefficient in running-in period decrease dramatically at the early stage of fretting wear.