A Method for the Determination of Atropine in Pharmaceutical Preparation Using CE-ECL

Abstract:

In this paper, a sensitive determination method for atropine based on end-column electrochemiluminescence of tris (2,2’-bipyridyl) ruthenium (II) detection is described. The conditions affecting separation and detection were investigated. Favorable ECL intensity of atropine was achieved in a solution consisting 5 mmol/L Ru (bpy)₃²⁺ and 50 mmol/L phosphate at applied voltage of 1.20V. The standard curve was linear between 1 and 20 μmol/L for atropine. The calibration equation and regression coefficients were: y = 128.38x−36.76 and R = 0.998 in terms of peak height response as a function of analyte concentration. A detection limit of 5×10⁻⁸ mol/L (S/N= 3) was achieved. The proposed method was applied satisfactorily to the determination of atropine in three pharmaceutical preparations.

• Periodical: Advanced Materials Research (Volumes 989-994)
• Pages: 1007-1010
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.989-994.1007
• Online since: July 2014
• Authors: Qian Xiang, Xiao Dong Yang*, Ying Gao
• Keywords: Atropine, Capillary Electrophoresis-Electrochemiluminescence (CE-ECL), Tris(2,2’-Bipyridyl)Ruthenium

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