Study on Degradation of Radix Isatidis Polypeptide in Artificial Gastrointestinal Environment

Abstract:

Radix Isatidis is a traditional Chinese medicine for treatment of influenza and inflammation in China. In this paper, in order to study the degradation situation of Radix Isatidis polypeptide in artificial gastrointestinal environment, the SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) method was used to detect the degradation of Radix Isatidis polypeptide in artificial intestinal juice and gastric juice, and it showed that Radix Isatidis peptides could be degradated to different degrees. HPLC (High Performance Liquid Chromatography) was used to determine the change of peptides degradation, and it indicated that free amino acid levels did not change significantly. The result after degradation was also detected by BCA method, and it showed that there were still a large number of polypeptides in the liquid. From this experiment we can come to this conclusion that Radix Isatidis polypeptides in artificial gastrointestinal juice mostly degraded into a series of different molecular weight peptides.

• Periodical: Advanced Materials Research (Volumes 989-994)
• Pages: 1020-1024
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.989-994.1020
• Online since: July 2014
• Authors: Nan Nan*, Xi Jing Liu
• Keywords: Artificial Gastric Juice, Artificial Intestinal Juice, Degradation, Radix Isatidis

References

[1] Sun Dong-dong, HE Li-wei and Chen Jian-wei et al. Optimization of extraction technology o Banlangen Radix isadix Isatidis based on multi-index comprehensive determination. Journal of Beijing University of traditional Chinese Medicine. 36(3): 183-187. (2013).

[2] Yang Feng-yun, Fu Ting-ming and Guo Li-wei, et al. Degradation of earthworm extracts prepared by wet superfine grinding in artificial gastrointestinal environment. Acta Pharmaceutica Sinica, 47(1): 110-115. (2012).

[3] Chen Kai, Dou Yue, Meng fan-gang et al. preliminary screening of anti-inflammatory fraction of isatidis radix[J]. Chinese Journal of Experimental Traditional Medieal formaulae. 18(6): 200-203. (2012).

[4] Zhu Ting, Yin Ruwen. Extract technology of total polysaccharides and active components effect from root of Isatis indigotica. Chinese Journal of New Drugs. 22(12): 1390-1395. (2013).

[5] Tang Jie, Wan Jin, Ma Yong-gui, et al. Research on the isolation and purification of organic acids from Radix Isatidis with macroporous adsorptive resin. Chin Hosp Pharm, 3(18): 1504. (2011).

[6] Xiao Hui, Lu Qing-fei, Wang Yi-ming. et al. Studies on extraction process of nucleosides for Radix Isatidis. Chinese Journal of New Drugs, 18(4): 353-356. (2009).

[7] Liu bo, Han xiaodong, Zhang Minting. Application AQC as a pre-column derivatization reagent for HPLC determination of free amino acids in borojo sorbilis cuter. ACTA scientiarum naturalium universtatis sunyatseni. 52(4): 100-104. (2013).