Nano-Fabricated Aligned Spheroid for Cartilage Tissue Engineering

Abstract:

Micropatterned PEGylated substrates with two-dimensional arrays of plasma-etched circular domains (diameter:100 micro-m) were prepared by coating of mercapto-functionalized poly(ethylene glycol) (PEG) on Au surface, followed by plasma-etching through a metal mask pattern with circular holes. The PEGylated region on the patterned substrate works to repel proteins, consequently, inhibits cell adhesion. Then the micro-patterning of bovine articular chondrocytes or rat primary hepatocytes hetero-spheroids underlaid with human umbilical endothelial cells (HUVEC) was achieved on the plasma-etched circular domains, exposing the base gold surface. Obtained results suggested that the efficiency of inhibiting non-specific protein adsorption significantly affects on construction of micro-patterned cell adhesion and hetero-spheroids. The formation of hetero-spheroid thus suggested is significantly modulated by suface properties, particularly non-fouling character of PEG region. These arrayed spheroids is promising materials for tissue and cell-based biosensors (TBB/CBB) as well as tissue engineering technologies.

• Periodical: Advances in Science and Technology (Volume 53)
• Pages: 67-69
• DOI: https://doi.org/10.4028/www.scientific.net/AST.53.67
• Online since: October 2006
• Authors: H. Otsuka, T. Satomi, Koji Ueno, Tetsuya Tateishi
• Keywords: Cartilage, Hepatocyte, Micro-Array, Micropatterning, Spheroid, Tissue Engineering

References

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